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dc.contributor.authorVan Der Hoek, K.-
dc.contributor.authorEyre, N.-
dc.contributor.authorShue, B.-
dc.contributor.authorKhantisitthiporn, O.-
dc.contributor.authorGlab-Ampi, K.-
dc.contributor.authorCarr, J.-
dc.contributor.authorGartner, M.-
dc.contributor.authorJolly, L.-
dc.contributor.authorThomas, P.-
dc.contributor.authorAdikusuma, F.-
dc.contributor.authorJankovic-Karasoulos, T.-
dc.contributor.authorRoberts, C.-
dc.contributor.authorHelbig, K.-
dc.contributor.authorBeard, M.-
dc.identifier.citationScientific Reports, 2017; 7(1):4475 -1-4475-14-
dc.descriptionPublished online 30 June 2017-
dc.description.abstractZika virus (ZIKV) infection has emerged as a global health threat and infection of pregnant women causes intrauterine growth restriction, spontaneous abortion and microcephaly in newborns. Here we show using biologically relevant cells of neural and placental origin that following ZIKV infection, there is attenuation of the cellular innate response characterised by reduced expression of IFN-β and associated interferon stimulated genes (ISGs). One such ISG is viperin that has well documented antiviral activity against a wide range of viruses. Expression of viperin in cultured cells resulted in significant impairment of ZIKV replication, while MEFs derived from CRISPR/Cas9 derived viperin-/- mice replicated ZIKV to higher titers compared to their WT counterparts. These results suggest that ZIKV can attenuate ISG expression to avoid the cellular antiviral innate response, thus allowing the virus to replicate unchecked. Moreover, we have identified that the ISG viperin has significant anti-ZIKV activity. Further understanding of how ZIKV perturbs the ISG response and the molecular mechanisms utilised by viperin to suppress ZIKV replication will aid in our understanding of ZIKV biology, pathogenesis and possible design of novel antiviral strategies.-
dc.description.statementofresponsibilityKylie H. Van der Hoek, Nicholas S. Eyre, Byron Shue, Onruedee Khantisitthiporn, Kittirat Glab-Ampi, Jillian M. Carr, Matthew J. Gartner, Lachlan A. Jolly, Paul Q. Thomas, Fatwa Adikusuma, Tanja Jankovic-Karasoulos, Claire T. Roberts, Karla J. Helbig and Michael R. Beard-
dc.publisherNature Publishing Group-
dc.rights© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectCell Line-
dc.subjectMice, Knockout-
dc.subjectDisease Models, Animal-
dc.subjectVirus Replication-
dc.subjectHost-Pathogen Interactions-
dc.subjectImmunity, Innate-
dc.subjectNeural Stem Cells-
dc.subjectCRISPR-Cas Systems-
dc.subjectZika Virus-
dc.subjectZika Virus Infection-
dc.subjectGene Editing-
dc.titleViperin is an important host restriction factor in control of Zika virus infection-
dc.typeJournal article-
dc.identifier.orcidVan Der Hoek, K. [0000-0002-7904-7340]-
dc.identifier.orcidEyre, N. [0000-0002-5424-7573]-
dc.identifier.orcidShue, B. [0000-0003-1686-1043]-
dc.identifier.orcidJolly, L. [0000-0003-4538-2658]-
dc.identifier.orcidAdikusuma, F. [0000-0003-2163-0514]-
dc.identifier.orcidRoberts, C. [0000-0002-9250-2192]-
dc.identifier.orcidBeard, M. [0000-0002-4106-1016]-
Appears in Collections:Aurora harvest 3
Paediatrics publications

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