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Type: Journal article
Title: Dynamic changes of small RNAs in rice spikelet development reveal specialized reproductive phasiRNA pathways
Author: Fei, Q.
Yang, L.
Liang, W.
Zhang, D.
Meyers, B.
Citation: Journal of Experimental Botany, 2016; 67(21):6037-6049
Publisher: Oxford University Press
Issue Date: 2016
ISSN: 0022-0957
Statement of
Qili Fei, Li Yang, Wanqi Liang, Dabing Zhang, Blake C. Meyers
Abstract: Dissection of the genetic pathways and mechanisms by which anther development occurs in grasses is crucial for both a basic understanding of plant development and for examining traits of agronomic importance such as male sterility. In rice, MULTIPLE SPOROCYTES1 (MSP1), a leucine-rich-repeat receptor kinase, plays an important role in anther development by limiting the number of sporocytes. OsTDL1a (a TPD1-like gene in rice) encodes a small protein that acts as a cofactor of MSP1 in the same regulatory pathway. In this study, we analyzed small RNA and mRNA changes in different stages of spikelets from wild-type rice, and from msp1 and ostdl1a mutants. Analysis of the small RNA data identified miRNAs demonstrating differential abundances. miR2275 was depleted in the two rice mutants; this miRNA is specifically enriched in anthers and functions to trigger the production of 24-nt phased secondary siRNAs (phasiRNAs) from PHAS loci. We observed that the 24-nt phasiRNAs as well as their precursor PHAS mRNAs were also depleted in the two mutants. An analysis of co-expression identified three Argonaute-encoding genes (OsAGO1d, OsAGO2b, and OsAGO18) that accumulate transcripts coordinately with phasiRNAs, suggesting a functional relationship. By mRNA in situ analysis, we demonstrated a strong correlation between the spatiotemporal pattern of these OsAGO transcripts and phasiRNA accumulations.
Keywords: Anther; Argonaute; microRNA; phasiRNA; rice; spikelet
Rights: © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030060015
DOI: 10.1093/jxb/erw361
Appears in Collections:Environment Institute publications

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