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Type: Journal article
Title: MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair in rice
Author: He, Y.
Wang, C.
Higgins, J.
Yu, J.
Zong, J.
Lu, P.
Zhang, D.
Liang, W.
Citation: The Plant Cell, 2016; 28(8):1879-1893
Publisher: American Society of Plant Biologists
Issue Date: 2016
ISSN: 1040-4651
Statement of
Yi He, Chong Wang, James D. Higgins, Junping Yu, Jie Zong, Pingli Lu, Dabing Zhang and Wanqi Liang
Abstract: F-box proteins constitute a large superfamily in plants and play important roles in controlling many biological processes, but the roles of F-box proteins in male meiosis in plants remain unclear. Here, we identify the rice (Oryza sativa) F-box gene MEIOTIC F-BOX (MOF), which is essential for male meiotic progression. MOF belongs to the FBX subfamily and is predominantly active during leptotene to pachytene of prophase I. mof meiocytes display disrupted telomere bouquet formation, impaired pairing and synapsis of homologous chromosomes, and arrested meiocytes at late prophase I, followed by apoptosis. Although normal, programmed double-stranded DNA breaks (DSBs) form in mof mutants, foci of the phosphorylated histone variant γH2AX, a marker for DSBs, persist in the mutant, indicating that many of the DSBs remained unrepaired. The recruitment of Completion of meiosis I (COM1) and Radiation sensitive51C (RAD51C) to DSBs is severely compromised in mutant meiocytes, indicating that MOF is crucial for DSB end-processing and repair. Further analyses showed that MOF could physically interact with the rice SKP1-like Protein1 (OSK1), indicating that MOF functions as a component of the SCF E3 ligase to regulate meiotic progression in rice. Thus, this study reveals the essential role of an F-box protein in plant meiosis and provides helpful information for elucidating the roles of the ubiquitin proteasome system in plant meiotic progression.
Keywords: F-Box Proteins
Cell Cycle Proteins
Plant Proteins
DNA Repair
DNA Breaks, Double-Stranded
Rights: © 2016 American Society of Plant Biologists. All rights reserved.
DOI: 10.1105/tpc.16.00108
Appears in Collections:Agriculture, Food and Wine publications
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