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dc.contributor.authorVeit, G.-
dc.contributor.authorOliver, K.-
dc.contributor.authorApaja, P.-
dc.contributor.authorPerdomo, D.-
dc.contributor.authorBidaud-Meynard, A.-
dc.contributor.authorLin, S.-
dc.contributor.authorGuo, J.-
dc.contributor.authorIcyuz, M.-
dc.contributor.authorSorscher, E.-
dc.contributor.authorHartman IV, J.-
dc.contributor.authorLukacs, G.-
dc.contributor.editorRon, D.-
dc.identifier.citationPLoS Biology, 2016; 14(5):e1002462-1-e1002462-32-
dc.description.abstractThe most common cystic fibrosis (CF) causing mutation, deletion of phenylalanine 508 (ΔF508 or Phe508del), results in functional expression defect of the CF transmembrane conductance regulator (CFTR) at the apical plasma membrane (PM) of secretory epithelia, which is attributed to the degradation of the misfolded channel at the endoplasmic reticulum (ER). Deletion of phenylalanine 670 (ΔF670) in the yeast oligomycin resistance 1 gene (YOR1, an ABC transporter) of Saccharomyces cerevisiae phenocopies the ΔF508-CFTR folding and trafficking defects. Genome-wide phenotypic (phenomic) analysis of the Yor1-ΔF670 biogenesis identified several modifier genes of mRNA processing and translation, which conferred oligomycin resistance to yeast. Silencing of orthologues of these candidate genes enhanced the ΔF508-CFTR functional expression at the apical PM in human CF bronchial epithelia. Although knockdown of RPL12, a component of the ribosomal stalk, attenuated the translational elongation rate, it increased the folding efficiency as well as the conformational stability of the ΔF508-CFTR, manifesting in 3-fold augmented PM density and function of the mutant. Combination of RPL12 knockdown with the corrector drug, VX-809 (lumacaftor) restored the mutant function to ~50% of the wild-type channel in primary CFTRΔF508/ΔF508 human bronchial epithelia. These results and the observation that silencing of other ribosomal stalk proteins partially rescue the loss-of-function phenotype of ΔF508-CFTR suggest that the ribosomal stalk modulates the folding efficiency of the mutant and is a potential therapeutic target for correction of the ΔF508-CFTR folding defect.-
dc.description.statementofresponsibilityGuido Veit, Kathryn Oliver, Pirjo M. Apaja, Doranda Perdomo, Aurélien Bidaud-Meynard, Sheng-Ting Lin, Jingyu Guo, Mert Icyuz, Eric J. Sorscher, John L. Hartman IV, Gergely L. Lukacs-
dc.publisherPublic Library of Science (PLoS)-
dc.rightsCopyright: © 2016 Veit et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited-
dc.subjectCells, Cultured-
dc.subjectEpithelial Cells-
dc.subjectCystic Fibrosis-
dc.subjectPeptide Elongation Factor 2-
dc.subjectATP-Binding Cassette Transporters-
dc.subjectCystic Fibrosis Transmembrane Conductance Regulator-
dc.subjectSaccharomyces cerevisiae Proteins-
dc.subjectRibosomal Proteins-
dc.subjectRNA, Small Interfering-
dc.subjectGene Silencing-
dc.subjectProtein Folding-
dc.subjectProtein Stability-
dc.subjectGene Knockdown Techniques-
dc.subjectHigh-Throughput Screening Assays-
dc.titleRibosomal stalk protein silencing partially corrects the ΔF508-CFTR functional expression defect-
dc.title.alternativeRibosomal stalk protein silencing partially corrects the DeltaF508-CFTR functional expression defect-
dc.typeJournal article-
dc.identifier.orcidApaja, P. [0000-0002-1622-2332]-
Appears in Collections:Aurora harvest 3
Molecular and Biomedical Science publications

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