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Type: Journal article
Title: Synthesis of complex oligosaccharides by using a mutated (1,3)-beta-D-glucan endohydrolase from barley
Author: Fairweather, J.
Hrmova, M.
Rutten, S.
Fincher, G.
Driguez, H.
Citation: Chemistry: A European Journal, 2003; 9(11):2603-2610
Publisher: Wiley-V C H Verlag GMBH
Issue Date: 2003
ISSN: 0947-6539
Statement of
Jon K. Fairweather, Maria Hrmova, Simon J. Rutten, Geoffrey B. Fincher, Hugues Driguez
Abstract: Complex oligosaccharides with newly formed (1,3)-β-glycosidic linkages were obtained in good to excellent yields when substituted or unsubstituted α-laminaribiosyl fluorides, acting as donors, were condensed onto mono- and disaccharide β-D-hexopyranoside acceptors by using a (1,3)-β-D-glycosynthase. These linear and branched (1,3)-β-linked oligosaccharides could prove to be important in a range of medical, pharmaceutical, and agricultural applications. Furthermore, the observation that the (1,3)-β-D-glucan glycosynthase accommodates (1,3)-, (1,4), - and (1,6)-β-oligosaccharides in its acceptor subsites suggests novel, yet unexpected physiological roles for the wild type (1,3)-β-D-glucan endohydrolase from higher plants.
Keywords: Carbohydrates
enzymatic synthesis
DOI: 10.1002/chem.200304733
Appears in Collections:Agriculture, Food and Wine publications
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