Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/109288
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Type: Journal article
Title: Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley
Author: Ferdous, J.
Whitford, R.
Nguyen, M.
Brien, C.
Langridge, P.
Tricker, P.
Citation: Functional and Integrative Genomics, 2017; 17(2-3):279-292
Publisher: Springer
Issue Date: 2017
ISSN: 1438-793X
1438-7948
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Responsibility: 
Jannatul Ferdous, Ryan Whitford, Martin Nguyen, Chris Brien, Peter Langridge, Penny J. Tricker
Abstract: Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic Arabidopsis. We examined barley (Hordeum vulgare L. 'Golden Promise') plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing CaMV-35S::Ath-miR827 and drought-inducible Zm-Rab17::Hv-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUEwp). We observed that the growth, WUEwp, time to anthesis and grain weight of transgenic barley plants expressing CaMV-35S::Ath-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing Zm-Rab17::Hv-miR827 showed improved WUEwp with no growth or reproductive timing change compared with the wild-type plants. The recovery of Zm-Rab17::Hv-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that Hv-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical.
Keywords: Ath-miR827; Hv-miR827; non-destructive imaging; promoter
Rights: © Springer-Verlag Berlin Heidelberg 2016
DOI: 10.1007/s10142-016-0526-8
Published version: http://dx.doi.org/10.1007/s10142-016-0526-8
Appears in Collections:Agriculture, Food and Wine publications
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