Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/110511
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dc.contributor.authorChai, M.en
dc.contributor.authorWeiland, F.en
dc.contributor.authorHarvey, R.en
dc.contributor.authorHoffmann, P.en
dc.contributor.authorOgunniyi, A.en
dc.contributor.authorPaton, J.en
dc.date.issued2017en
dc.identifier.citationScientific Reports, 2017; 7(1):2453-1-2453-11en
dc.identifier.issn2045-2322en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://hdl.handle.net/2440/110511-
dc.description.abstractStreptococcus pneumoniae (the pneumococcus) is a human pathogen, accounting for massive global morbidity and mortality. Although asymptomatic colonization of the nasopharynx almost invariably precedes disease, the critical determinants enabling pneumococcal progression from this niche to cause invasive disease are poorly understood. One mechanism proposed to be central to this transition involves opacity phase variation, whereby pneumococci harvested from the nasopharynx are typically transparent, while those simultaneously harvested from the blood are opaque. Here, we used two dimensional-differential gel electrophoresis (2D-DIGE) to compare protein expression profiles of transparent and opaque variants of 3 pneumococcal strains, D39 (serotype 2), WCH43 (serotype 4) and WCH16 (serotype 6A) in vitro. One spot comprising a mixture of capsular polysaccharide biosynthesis protein and other proteins was significantly up-regulated in the opaque phenotype in all 3 strains; other proteins were differentially regulated in a strain-specific manner. We conclude that pneumococcal phase variation is a complex and multifactorial process leading to strain-specific pathogenicity.en
dc.description.statementofresponsibilityMelissa H. Chai, Florian Weiland, Richard M. Harvey, Peter Hoffmann, Abiodun D. Ogunniyi, James C. Patonen
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectNasopharynx; Humans; Streptococcus pneumoniae; Pneumococcal Infections; Bacterial Proteins; Blotting, Western; Electrophoresis, Gel, Two-Dimensional; Serotyping; Proteomics; Host-Pathogen Interactions; Serogroupen
dc.titleProteomic comparisons of opaque and transparent variants of Streptococcus pneumoniae by two dimensional-differential gel electrophoresisen
dc.typeJournal articleen
dc.identifier.rmid0030070427en
dc.identifier.doi10.1038/s41598-017-02465-xen
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/565526en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/627142en
dc.identifier.pubid355297-
pubs.library.collectionAnimal and Veterinary Sciences publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidWeiland, F. [0000-0001-9702-9245]en
dc.identifier.orcidOgunniyi, A. [0000-0001-9308-5629]en
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]en
Appears in Collections:Animal and Veterinary Sciences publications

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