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Type: Journal article
Title: A genetic screen for impaired systemic RNAi highlights the crucial role of DICER-LIKE 2
Author: Taochy, C.
Gursanscky, N.
Cao, J.
Fletcher, S.
Dressel, U.
Mitter, N.
Tucker, M.
Koltunow, A.
Bowman, J.
Vaucheret, H.
Carroll, B.
Citation: Plant physiology, 2017; 175(3):1424-1437
Publisher: American Society of Plant Biologists
Issue Date: 2017
ISSN: 0032-0889
Statement of
Christelle Taochy, Nial R. Gursanscky, Jiangling Cao, Stephen J. Fletcher, Uwe Dressel, Neena Mitter, Matthew R. Tucker, Anna M.G. Koltunow, John L. Bowman, Hervé Vaucheret and Bernard J. Carroll
Abstract: Post-transcriptional gene silencing (PTGS) of transgenes involves abundant 21 nt small interfering RNAs (siRNAs) and low abundance 22 nt siRNAs produced from double-stranded RNA (dsRNA) by DCL4 and DCL2, respectively. However, DCL2 facilitates the recruitment of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1 (AGO1)-derived cleavage products, resulting in more efficient amplification of secondary and transitive dsRNA and siRNAs. Here, we describe a reporter system where RDR6-dependent PTGS is initiated by restricted expression of an inverted-repeat dsRNA specifically in the root tip, allowing a genetic screen to identify mutants impaired in RDR6-dependent systemic PTGS. Our screen identified dcl2 but not dcl4 mutants. Moreover, grafting experiments showed that DCL2, but not DCL4, is required in both the source rootstock and recipient shoot tissue for efficient RDR6-dependent systemic PTGS. Furthermore, dcl4 rootstocks produced more DCL2-dependent 22 nt siRNAs than wild type and showed enhanced systemic movement of PTGS to grafted shoots. Thus, along with its role in recruiting RDR6 for further amplification of PTGS, DCL2 is crucial for RDR6-dependent systemic PTGS.
Keywords: Arabidopsis; Plant Shoots; Plant Roots; Ribonuclease III; Glucuronidase; RNA Replicase; Cell Cycle Proteins; Green Fluorescent Proteins; Arabidopsis Proteins; RNA, Small Interfering; RNA Interference; Phenotype; Mutation; Genes, Reporter; Models, Biological; Genetic Testing
Rights: © 2017 American Society of Plant Biologists. All Rights Reserved.
RMID: 0030076164
DOI: 10.1104/pp.17.01181
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Appears in Collections:Agriculture, Food and Wine publications

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