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Type: Journal article
Title: Salinity effects on chloroplast PSII performance in glycophytes and halophytes
Author: Percey, W.
McMinn, A.
Bose, J.
Breadmore, M.
Guijt, R.
Shabala, S.
Citation: Functional Plant Biology: an international journal of plant function, 2016; 43(11):1003-1015
Publisher: CSIRO Publishing
Issue Date: 2016
ISSN: 1445-4408
Statement of
William J. Percey, Andrew McMinn, Jayakumar Bose, Michael C. Breadmore, Rosanne M. Guijt and Sergey Shabala
Abstract: The effects of NaCl stress and K⁺ nutrition on photosynthetic parameters of isolated chloroplasts were investigated using PAM fluorescence. Intact mesophyll cells were able to maintain optimal photosynthetic performance when exposed to salinity for more than 24 h whereas isolated chloroplasts showed declines in both the relative electron transport rate (rETR) and the maximal photochemical efficiency of PSII (Fv/Fm) within the first hour of treatment. The rETR was much more sensitive to salt stress compared with Fv/Fm, with 40% inhibition of rETR observed at apoplastic NaCl concentration as low as 20 mM. In isolated chloroplasts, absolute K⁺ concentrations were more essential for the maintenance of the optimal photochemical performance (Fv/Fm values) rather than sodium concentrations per se. Chloroplasts from halophyte species of quinoa (Chenopodium quinoa Willd.) and pigface (Carpobrotus rosii (Haw.) Schwantes) showed less than 18% decline in Fv/Fm under salinity, whereas the Fv/Fm decline in chloroplasts from glycophyte pea (Pisum sativum L.) and bean (Vicia faba L.) species was much stronger (31 and 47% respectively). Vanadate (a P-type ATPase inhibitor) significantly reduced Fv/Fm in both control and salinity treated chloroplasts (by 7 and 25% respectively), whereas no significant effects of gadolinium (blocker of non-selective cation channels) were observed in salt-treated chloroplasts. Tetraethyl ammonium (TEA) (K⁺ channel inhibitor) and amiloride (inhibitor of the Na⁺/H⁺ antiporter) increased the Fv/Fm of salinity treated chloroplasts by 16 and 17% respectively. These results suggest that chloroplasts’ ability to regulate ion transport across the envelope and thylakoid membranes play a critical role in leaf photosynthetic performance under salinity.
Keywords: Membrane transport; non-stomatal limitation; photosynthesis; potassium; ROS; sodium
Rights: Journal compilation © CSIRO 2016
DOI: 10.1071/FP16135
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Appears in Collections:Agriculture, Food and Wine publications
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