Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/111095
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Type: Journal article
Title: An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing
Author: Purdey, M.
Capon, P.
Pullen, B.
Reineck, P.
Schwarz, N.
Psaltis, P.
Nicholls, S.
Gibson, B.
Abell, A.
Citation: Scientific Reports, 2017; 7(1):15967-1-15967-8
Publisher: Nature Publishing Group
Issue Date: 2017
ISSN: 2045-2322
2045-2322
Statement of
Responsibility: 
Malcolm S. Purdey, Patrick K. Capon, Benjamin J. Pullen, Philipp Reineck, Nisha Schwarz, Peter J. Psaltis, Stephen J. Nicholls, Brant C. Gibson and Andrew D. Abell
Abstract: Organic fluorescent probes are widely used to detect key biomolecules; however, they often lack the photostability required for extended intracellular imaging. Here we report a new hybrid nanomaterial (peroxynanosensor, PNS), consisting of an organic fluorescent probe bound to a nanodiamond, that overcomes this limitation to allow concurrent and extended cell-based imaging of the nanodiamond and ratiometric detection of hydrogen peroxide. Far-red fluorescence of the nanodiamond offers continuous monitoring without photobleaching, while the green fluorescence of the organic fluorescent probe attached to the nanodiamond surface detects hydrogen peroxide on demand. PNS detects basal production of hydrogen peroxide within M1 polarised macrophages and does not affect macrophage growth during prolonged co-incubation. This nanosensor can be used for extended bio-imaging not previously possible with an organic fluorescent probe, and is spectrally compatible with both Hoechst 33342 and MitoTracker Orange stains for hyperspectral imaging.
Keywords: Cell Nucleus; Macrophages; Animals; Mice, Inbred C57BL; Fluorescent Dyes; Imaging, Three-Dimensional; Biosensing Techniques; Photobleaching; Light; Fluorescence; Nanodiamonds
Rights: © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
RMID: 0030077841
DOI: 10.1038/s41598-017-15772-0
Grant ID: http://purl.org/au-research/grants/arc/CE140100003
Appears in Collections:Physics publications

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