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https://hdl.handle.net/2440/111112
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Type: | Journal article |
Title: | Photoswitchable calcium sensor: ‘On’–‘Off’ sensing in cells or with microstructured optical fibers |
Author: | Heng, S. Mak, A. Kostecki, R. Zhang, X. Pei, J. Stubing, D. Ebendorff-Heidepriem, H. Abell, A. |
Citation: | Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2017; 252:965-972 |
Publisher: | Elsevier |
Issue Date: | 2017 |
ISSN: | 0925-4005 0925-4005 |
Statement of Responsibility: | Sabrina Heng, Adrian M. Mak, Roman Kostecki, Xiaozhou Zhang Jinxin Pei, Daniel B.Stubing, Heike Ebendorff-Heidepriem, Andrew D.Abell |
Abstract: | Calcium is a ubiquitous intracellular signaling ion that plays a critical role in the modulation of fundamental cellular processes. A detailed study of these processes requires selective and reversible sensing of Ca2+ and an ability to quantify and monitor concentration changes in a biological setting. Three new, rationally designed, synthesized and photoswitchable spiropyran-based reversible sensors for Ca2+ are reported. Sensor 1a is highly selective for Ca2+ with an improved profile relative to the other two analogues, 1b and 1c. Formation of the merocyanine–Ca2+ complex is proportional to an increase in Ca2+ released from HEK293 cells on stimulation with ionomycin. The photophysical processes surrounding the binding of Ca2+ to compound 1a were further explored using computational methods based on density functional theory (DFT). The ability of sensor 1a to bind Ca2+ and photoswitch reversibly was also characterized using silica suspended-core microstructured optical fiber (SCF). These SCF experiments (with 100 nM Ca2+) represent a first step toward developing photoswitchable, minimally invasive and highly sensitive Ca2+ sensing platforms for use in a biological setting. |
Keywords: | Spiropyran; calcium sensing; density functional theory; suspended-core microstructured optical fiber |
Rights: | © 2017 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.snb.2017.06.051 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100003 |
Published version: | http://dx.doi.org/10.1016/j.snb.2017.06.051 |
Appears in Collections: | Aurora harvest 3 IPAS publications |
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