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|Title:||Photoswitchable calcium sensor: ‘On’–‘Off’ sensing in cells or with microstructured optical fibers|
|Citation:||Sensors and Actuators, B: Chemical, 2017; 252:965-972|
|Sabrina Heng, Adrian M. Mak, Roman Kostecki, Xiaozhou Zhang Jinxin Pei, Daniel B.Stubing, Heike Ebendorff-Heidepriem, Andrew D.Abell|
|Abstract:||Calcium is a ubiquitous intracellular signaling ion that plays a critical role in the modulation of fundamental cellular processes. A detailed study of these processes requires selective and reversible sensing of Ca2+ and an ability to quantify and monitor concentration changes in a biological setting. Three new, rationally designed, synthesized and photoswitchable spiropyran-based reversible sensors for Ca2+ are reported. Sensor 1a is highly selective for Ca2+ with an improved profile relative to the other two analogues, 1b and 1c. Formation of the merocyanine–Ca2+ complex is proportional to an increase in Ca2+ released from HEK293 cells on stimulation with ionomycin. The photophysical processes surrounding the binding of Ca2+ to compound 1a were further explored using computational methods based on density functional theory (DFT). The ability of sensor 1a to bind Ca2+ and photoswitch reversibly was also characterized using silica suspended-core microstructured optical fiber (SCF). These SCF experiments (with 100 nM Ca2+) represent a first step toward developing photoswitchable, minimally invasive and highly sensitive Ca2+ sensing platforms for use in a biological setting.|
|Keywords:||Spiropyran; calcium sensing; density functional theory; suspended-core microstructured optical fiber|
|Rights:||© 2017 Elsevier B.V. All rights reserved.|
|Appears in Collections:||IPAS publications|
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