Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/111290
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Type: Journal article
Title: A method for non-invasive genotyping of APC(min+) mice using fecal samples
Author: Symonds, E.
Fenech, M.
Citation: Biological Procedures Online, 2012; 14(1):1-3
Publisher: BioMed Central
Issue Date: 2012
ISSN: 1480-9222
1480-9222
Statement of
Responsibility: 
Erin L Symonds and Michael Fenech
Abstract: The APC(min/+) mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.
Keywords: APC(min/+); feces; genotyping; cancer; non-invasive
Rights: © 2012 Symonds and Fenech; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030081613
DOI: 10.1186/1480-9222-14-1
Grant ID: http://purl.org/au-research/grants/nhmrc/357702
Appears in Collections:Medicine publications

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