Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/111393
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Type: | Journal article |
Title: | Genotoxicity and cytotoxicity of the plasma jet-treated medium on lymphoblastoid WIL2-NS cell line using the cytokinesis block micronucleus cytome assay |
Author: | Hong, S.-H. Szili, E.J. Fenech, M. Gaur, N. Short, R.D. |
Citation: | Scientific Reports, 2017; 7(1):3854-1-3854-9 |
Publisher: | Nature Publishing Group |
Issue Date: | 2017 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Sung-Ha Hong, Endre J. Szili, Michael Fenech, Nishtha Gaur, Robert D. Short |
Abstract: | Despite growing interest in the application of atmospheric plasma jets as medical treatment strategies, there has been comparatively little research on the potential genotoxic and cytotoxic effects of plasma jet treatment. In this study, we have employed the cytokinesis block micronucleus cytome (CBMN-Cyt) assay with WIL2-NS B lymphoblastoid cells to test the potential genotoxicity, as well as the cytotoxicity, of toxic species generated in cell culture media by an argon (Ar) plasma jet. Elevated levels of cell death (necrosis) and occurrence of chromosomal damage (micronuclei MN, nculeoplasmic bridge NPBs and nuclear bus, Nbuds) were observed when cells were exposed to plasma jet-treated media. These results provide a first insight into how we might measure the genotoxic and cytotoxic effect of plasma jet treatments (both indirect and direct) in dividing human cells. |
Keywords: | Lymphocytes Cell Line Humans Argon Culture Media, Conditioned Analysis of Variance Micronucleus Tests Cytokinesis |
Rights: | © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
DOI: | 10.1038/s41598-017-03754-1 |
Grant ID: | http://purl.org/au-research/grants/arc/DP160104298 |
Published version: | http://dx.doi.org/10.1038/s41598-017-03754-1 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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hdl_111393.pdf | Published version | 1.56 MB | Adobe PDF | View/Open |
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