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Type: Journal article
Title: Genotoxicity and cytotoxicity of the plasma jet-treated medium on lymphoblastoid WIL2-NS cell line using the cytokinesis block micronucleus cytome assay
Author: Hong, S.-.H.
Szili, E.
Fenech, M.
Gaur, N.
Short, R.
Citation: Scientific Reports, 2017; 7(1):3854-1-3854-9
Publisher: Nature Publishing Group
Issue Date: 2017
ISSN: 2045-2322
Statement of
Sung-Ha Hong, Endre J. Szili, Michael Fenech, Nishtha Gaur, Robert D. Short
Abstract: Despite growing interest in the application of atmospheric plasma jets as medical treatment strategies, there has been comparatively little research on the potential genotoxic and cytotoxic effects of plasma jet treatment. In this study, we have employed the cytokinesis block micronucleus cytome (CBMN-Cyt) assay with WIL2-NS B lymphoblastoid cells to test the potential genotoxicity, as well as the cytotoxicity, of toxic species generated in cell culture media by an argon (Ar) plasma jet. Elevated levels of cell death (necrosis) and occurrence of chromosomal damage (micronuclei MN, nculeoplasmic bridge NPBs and nuclear bus, Nbuds) were observed when cells were exposed to plasma jet-treated media. These results provide a first insight into how we might measure the genotoxic and cytotoxic effect of plasma jet treatments (both indirect and direct) in dividing human cells.
Keywords: Lymphocytes; Cell Line; Humans; Argon; Culture Media, Conditioned; Analysis of Variance; Micronucleus Tests; Cytokinesis
Rights: © The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
RMID: 0030081571
DOI: 10.1038/s41598-017-03754-1
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Appears in Collections:Medicine publications

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