Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/111669
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dc.contributor.authorXu, B.en
dc.contributor.authorWaters, S.en
dc.contributor.authorByrt, C.en
dc.contributor.authorPlett, D.en
dc.contributor.authorTyerman, S.en
dc.contributor.authorTester, M.en
dc.contributor.authorMunns, R.en
dc.contributor.authorHrmova, M.en
dc.contributor.authorGilliham, M.en
dc.date.issued2018en
dc.identifier.citationCellular and Molecular Life Sciences, 2018; 75(6):1133-1144en
dc.identifier.issn1420-682Xen
dc.identifier.issn1420-9071en
dc.identifier.urihttp://hdl.handle.net/2440/111669-
dc.description.abstractAn important trait associated with the salt tolerance of wheat is the exclusion of sodium ions ( Na⁺) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na⁺- exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na⁺ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D⁴⁷¹/a gap and D⁴⁷⁴/ G⁴⁷³ that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na⁺ exclusion in wheat that leads to an improved salinity tolerance in the field.en
dc.description.statementofresponsibilityBo Xu, Shane Waters, Caitlin S. Byrt, Darren Plett, Stephen D. Tyerman, Mark Tester, Rana Munns, Maria Hrmova, Matthew Gillihamen
dc.language.isoenen
dc.publisherSpringeren
dc.rights© Springer International Publishing AG, part of Springer Nature 2017en
dc.subjectGatekeeper cells; salt exclusion; ion transport; structure-function; einkorn; bread; salt tolerance; Xenopus; mutagenesis; yeast; high-affinity K⁺ transporteren
dc.titleStructural variations in wheat HKT1;5 underpin differences in Na+ transport capacityen
dc.typeJournal articleen
dc.identifier.rmid0030078267en
dc.identifier.doi10.1007/s00018-017-2716-5en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP120100900en
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100008en
dc.relation.granthttp://purl.org/au-research/grants/arc/FT130100709en
dc.relation.granthttp://purl.org/au-research/grants/arc/DE150100837en
dc.identifier.pubid387429-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Agriculture, Food and Wine publications

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