Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/111669
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DC Field | Value | Language |
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dc.contributor.author | Xu, B. | - |
dc.contributor.author | Waters, S. | - |
dc.contributor.author | Byrt, C. | - |
dc.contributor.author | Plett, D. | - |
dc.contributor.author | Tyerman, S. | - |
dc.contributor.author | Tester, M. | - |
dc.contributor.author | Munns, R. | - |
dc.contributor.author | Hrmova, M. | - |
dc.contributor.author | Gilliham, M. | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Cellular and Molecular Life Sciences, 2018; 75(6):1133-1144 | - |
dc.identifier.issn | 1420-682X | - |
dc.identifier.issn | 1420-9071 | - |
dc.identifier.uri | http://hdl.handle.net/2440/111669 | - |
dc.description.abstract | An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions ( Na⁺) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na⁺- exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na⁺ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D⁴⁷¹/a gap and D⁴⁷⁴/ G⁴⁷³ that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na⁺ exclusion in wheat that leads to an improved salinity tolerance in the field. | - |
dc.description.statementofresponsibility | Bo Xu, Shane Waters, Caitlin S. Byrt, Darren Plett, Stephen D. Tyerman, Mark Tester, Rana Munns, Maria Hrmova, Matthew Gilliham | - |
dc.language.iso | en | - |
dc.publisher | Springer | - |
dc.rights | © Springer International Publishing AG, part of Springer Nature 2017 | - |
dc.source.uri | http://dx.doi.org/10.1007/s00018-017-2716-5 | - |
dc.subject | Gatekeeper cells; salt exclusion; ion transport; structure-function; einkorn; bread; salt tolerance; Xenopus; mutagenesis; yeast; high-affinity K⁺ transporter | - |
dc.title | Structural variations in wheat HKT1;5 underpin differences in Na+ transport capacity | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1007/s00018-017-2716-5 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP120100900 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100008 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FT130100709 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DE150100837 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Xu, B. [0000-0002-7583-2384] | - |
dc.identifier.orcid | Byrt, C. [0000-0001-8549-2873] | - |
dc.identifier.orcid | Plett, D. [0000-0002-9551-8755] | - |
dc.identifier.orcid | Tyerman, S. [0000-0003-2455-1643] | - |
dc.identifier.orcid | Hrmova, M. [0000-0002-3545-0605] | - |
dc.identifier.orcid | Gilliham, M. [0000-0003-0666-3078] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
Files in This Item:
File | Description | Size | Format | |
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hdl_111669.pdf | Submitted Version | 1.81 MB | Adobe PDF | View/Open |
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