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Type: Journal article
Title: The use of CRISPR/Cas9 gene editing to confirm congenic contaminations in host-pathogen interaction studies
Author: Ferrand, J.
Croft, N.
Pépin, G.
Diener, K.
Wu, D.
Mangan, N.
Pedersen, J.
Behlke, M.
Hayball, J.
Purcell, A.
Ferrero, R.
Gantier, M.
Citation: Frontiers in Cellular and Infection Microbiology, 2018; 8(MAR):87-1-87-10
Publisher: Frontiers Media SA
Issue Date: 2018
ISSN: 2235-2988
Statement of
Jonathan Ferrand, Nathan P. Croft, Geneviève Pépin, Kerrilyn R. Diener, Di Wu, Niamh E. Mangan, John Pedersen, Mark A. Behlke, John D. Hayball, Anthony W. Purcell, Richard L. Ferrero, and Michael P. Gantier
Abstract: Murine models of Salmonella enterica serovar Typhimurium infection are one of the commonest tools to study host-pathogen interactions during bacterial infections. Critically, the outcome of S. Typhimurium infection is impacted by the genetic background of the mouse strain used, with macrophages from C57BL/6 and BALB/c mice lacking the capacity to control intracellular bacterial replication. For this reason, the use of congenic strains, which mix the genetic backgrounds of naturally protected mouse strains with those of susceptible strains, has the capacity to significantly alter results and interpretation of S. Typhimurium infection studies. Here, we describe how macrophage knockout cell lines generated by CRISPR/Cas9 gene editing can help determine the contribution of background contaminations in the phenotypes of primary macrophages from congenic mice, on the outcome of S. Typhimurium infection studies. Our own experience illustrates how the CRISPR/Cas9 technology can be used to complement pre-existing knockout models, and shows that there is great merit in performing concurrent studies with both genetic models, to exclude unanticipated side-effects on host-pathogen interactions.
Keywords: Salmonella; CRISPR/CAS9; congenic mice; background contamination; host-pathogen interactions
Rights: Copyright © 2018 Ferrand, Croft, Pépin, Diener, Wu, Mangan, Pedersen, Behlke, Hayball, Purcell, Ferrero and Gantier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI: 10.3389/fcimb.2018.00087
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