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Type: Journal article
Title: Structural and functional characterisation of the cytochrome P450 enzyme CYP268A2 from Mycobacterium marinum
Author: Child, S.
Naumann, E.
Bruning, J.
Bell, S.
Citation: The Biochemical Journal, 2018; 475(4):705-722
Publisher: Portland Press
Issue Date: 2018
ISSN: 0264-6021
Statement of
Stella A. Child, Elise F. Naumann, John B. Bruning and Stephen G. Bell
Abstract: Members of the cytochrome P450 monooxygenase family CYP268 are found across a broad range of Mycobacterium species including the pathogens Mycobacterium avium, M. colombiense,M. kansasii and Mmarinum CYP268A2, from M. marinum , which is the first member of this family to be studied, was purified and characterised. CYP268A2 was found to bind a variety of substrates with high affinity, including branched and straight-chain fatty acids (C10-C12), acetate esters, and aromatic compounds. The enzyme was also found to bind phenylimidazole inhibitors but not larger azoles, such as ketoconazole. The monooxygenase activity of CYP268A2 was efficiently reconstituted using heterologous electron transfer partner proteins. CYP268A2 hydroxylated geranyl acetate and tran s-pseudoionone at a terminal methyl group to yield ( 2E,6E )-8-hydroxy-3,7-dimethylocta-2,6-dien-1-yl acetate and ( 3E,5E,9E )-11-hydroxy-6,10-dimethylundeca-3,5,9-trien-2-one, respectively. The X-ray crystal structure of CYP268A2 was solved to a resolution of 2.0 Å with trans-pseudoionone bound in the active site. The overall structure was similar to that of the related phytanic acid monooxygenase CYP124A1 enzyme from Mycobacterium tuberculosis , which shares 41 % sequence identity. The active site is predominantly hydrophobic but includes the Ser99 and Gln209 residues which form hydrogen bonds with the terminal carbonyl group of the pseudoionone. The structure provided an explanation on why CYP268A2 shows a preference for shorter substrates over the longer chain fatty acids which bind to CYP124A1 and the selective nature of the catalysed monooxygenase activity.
Keywords: Crystallography; cytochrome P450; monooxygenases; Mycobacterium
Rights: © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
RMID: 0030081311
DOI: 10.1042/bcj20170946
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Appears in Collections:IPAS publications
Chemistry publications

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