Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/111835
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Type: Journal article
Title: An in vivo method to quantify lymphangiogenesis in zebrafish
Author: Hoffman, S.
Psaltis, P.
Clark, K.
Spoon, D.
Chue, C.
Ekker, S.
Simari, R.
Citation: PLoS ONE, 2012; 7(9):e45240-1-e45240-8
Publisher: Public Library of Science
Issue Date: 2012
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Scott J. Hoffman, Peter J. Psaltis, Karl J. Clark, Daniel B. Spoon, Colin D. Chue, Stephen C. Ekker, Robert D. Simari
Abstract: Lymphangiogenesis is a highly regulated process involved in the pathogenesis of disease. Current in vivo models to assess lymphangiogenesis are largely unphysiologic. The zebrafish is a powerful model system for studying development, due to its rapid growth and transparency during early stages of life. Identification of a network of trunk lymphatic capillaries in zebrafish provides an opportunity to quantify lymphatic growth in vivo.Late-phase microangiography was used to detect trunk lymphatic capillaries in zebrafish 2- and 3-days post-fertilization. Using this approach, real-time changes in lymphatic capillary development were measured in response to modulators of lymphangiogenesis. Recombinant human vascular endothelial growth factor (VEGF)-C added directly to the zebrafish aqueous environment as well as human endothelial and mouse melanoma cell transplantation resulted in increased lymphatic capillary growth, while morpholino-based knockdown of vegfc and chemical inhibitors of lymphangiogenesis added to the aqueous environment resulted in decreased lymphatic capillary growth.Lymphatic capillaries in embryonic and larval zebrafish can be quantified using late-phase microangiography. Human activators and small molecule inhibitors of lymphangiogenesis, as well as transplanted human endothelial and mouse melanoma cells, alter lymphatic capillary development in zebrafish. The ability to rapidly quantify changes in lymphatic growth under physiologic conditions will allow for broad screening of lymphangiogenesis modulators, as well as help define cellular roles and elucidate pathways of lymphatic development.
Keywords: Zebrafish; fluorescence imaging; capillaries; morpholino; melanoma cells; blood vessels; embryos; endothelial cells
Rights: © Hoffman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030063182
DOI: 10.1371/journal.pone.0045240
Appears in Collections:Medicine publications

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