Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/112431
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dc.contributor.authorWilliams, J.en
dc.contributor.authorIamartino, D.en
dc.contributor.authorPruitt, K.en
dc.contributor.authorSonstegard, T.en
dc.contributor.authorSmith, T.en
dc.contributor.authorLow, W.en
dc.contributor.authorBiagini, T.en
dc.contributor.authorBomba, L.en
dc.contributor.authorCapomaccio, S.en
dc.contributor.authorCastiglioni, B.en
dc.contributor.authorColetta, A.en
dc.contributor.authorCorrado, F.en
dc.contributor.authorFerré, F.en
dc.contributor.authorIannuzzi, L.en
dc.contributor.authorLawley, C.en
dc.contributor.authorMacciotta, N.en
dc.contributor.authorMcClure, M.en
dc.contributor.authorMancini, G.en
dc.contributor.authorMatassino, D.en
dc.contributor.authorMazza, R.en
dc.contributor.authoret al.en
dc.date.issued2017en
dc.identifier.citationGigaScience, 2017; 6(10):1-6en
dc.identifier.issn2047-217Xen
dc.identifier.issn2047-217Xen
dc.identifier.urihttp://hdl.handle.net/2440/112431-
dc.description.abstractWater buffalo is a globally important species for agriculture and local economies. A de novo assembled, well annotated, reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. However, no such genome assembly has been previously reported. There are two species of domestic water buffalo, the river (2n = 50) and the swamp (2n = 48) buffalo. Here we describe a draft quality reference sequence for the river buffalo created from Illumina GA and Roche 454 short read sequences using the MaSuRCA assembler. The assembled sequence is 2.83 Gb, consisting of 366,983 scaffolds with a scaffold N50 of 1.41 Mb and contig N50 of 21,398 bp. Annotation of the genome was supported by transcriptome data from 30 tissues, and identified 21,711 predicted protein coding genes. Searches for complete mammalian BUSCO gene groups found 98.6% of curated single copy orthologs present among predicted genes, which suggests a high level of completeness of the genome. The annotated sequence is available from NCBI at accession GCA_000471725.1.en
dc.description.statementofresponsibilityJohn L. Williams, Daniela Iamartino, Kim D. Pruitt, Tad Sonstegard, Timothy P.L. Smith, Wai Yee Low, Tommaso Biagini, Lorenzo Bomba, Stefano Capomaccio, Bianca Castiglioni, Angelo Coletta, Federica Corrado, Fabrizio Ferré, Leopoldo Iannuzzi, Cynthia Lawley, Nicolò Macciotta, Matthew McClure, Giordano Mancini, Donato Matassino, Raffaele Mazza, Marco Milanesi, Bianca Moioli, Nicola Morandi, Luigi Ramunno, Vincenzo Peretti, Fabio Pilla, Paola Ramelli, Steven Schroeder, Francesco Strozzi, Francoise Thibaud-Nissen, Luigi Zicarelli, Paolo Ajmone-Marsan, Alessio Valentini, Giovanni Chillemi, and Aleksey Ziminen
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rights© The Author 2017. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectWater buffalo; genome assembly; transcriptome; annotationen
dc.titleGenome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n = 50)en
dc.typeJournal articleen
dc.identifier.rmid0030075951en
dc.identifier.doi10.1093/gigascience/gix088en
dc.identifier.pubid370485-
pubs.library.collectionAnimal and Veterinary Sciences publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidWilliams, J. [0000-0001-5188-7957]en
dc.identifier.orcidLow, W. [0000-0002-0749-765X]en
Appears in Collections:Animal and Veterinary Sciences publications

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