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Type: Journal article
Title: Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation
Author: Schlötterer, C.
Kofler, R.
Versace, E.
Tobler, R.
Franssen, S.
Citation: Heredity, 2015; 114(5):431-440
Publisher: Springer Nature
Issue Date: 2015
ISSN: 0018-067X
Statement of
C Schlötterer, R Kofler, E Versace, R Tobler and SU Franssen
Abstract: Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype–phenotype link and serve as an important research tool for a broad range of evolutionary questions.
Keywords: Animals
Drosophila melanogaster
Adaptation, Physiological
Gene Frequency
Polymorphism, Single Nucleotide
Research Design
Genetic Variation
Genetic Association Studies
Biological Evolution
High-Throughput Nucleotide Sequencing
Rights: © 2015 Macmillan Publishers Limited All rights reserved This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
DOI: 10.1038/hdy.2014.86
Appears in Collections:Aurora harvest 3
Genetics publications

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