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Type: Journal article
Title: Resolvase OsGEN1 mediates DNA repair by homologous recombination
Author: Wang, C.
Higgins, J.
He, Y.
Lu, P.
Zhang, D.
Liang, W.
Citation: Plant Physiology, 2017; 173(2):1316-1329
Publisher: American Society of Plant Biologists
Issue Date: 2017
ISSN: 0032-0889
Statement of
Chong Wang, James D. Higgins, Yi He, Pingli Lu, Dabing Zhang, Wanqi Liang
Abstract: Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. In eukaryotes, such as budding yeast, mice, worms, and humans, Yen1/GEN1 work together with Mus81-Mms4/MUS81-EME1 and Slx1-Slx4/SLX1-SLX4 in DNA repair by homologous recombination to maintain genome stability. In plants, the biological function of Yen1/GEN1 remains largely unclear. In this study, we characterized the loss of function mutants of OsGEN1 and OsSEND1, a pair of paralogs of Yen1/GEN1 in rice (Oryza sativa). We first investigated the role of OsGEN1 during meiosis and found a reduction in chiasma frequency by ∼6% in osgen1 mutants, compared to the wild type, suggesting a possible involvement of OsGEN1 in the formation of crossovers. Postmeiosis, OsGEN1 foci were detected in wild-type microspore nuclei, but not in the osgen1 mutant concomitant with an increase in double-strand breaks. Persistent double-strand breaks led to programmed cell death of the male gametes and complete male sterility. In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance osgen1 defects. Our results indicate that OsGEN1 is essential for homologous recombinational DNA repair at two stages of microsporogenesis in rice.
Keywords: Chromosomes, Plant
Synaptonemal Complex
Plants, Genetically Modified
Plant Proteins
Recombinant Proteins
DNA Repair
Homologous Recombination
Rights: © 2017 American Society of Plant Biologists. All Rights Reserved.
DOI: 10.1104/pp.16.01726
Appears in Collections:Agriculture, Food and Wine publications
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