Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113086
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Type: Journal article
Title: Bronchiolitis obliterans syndrome is associated with increased p-glycoprotein expression and loss of glucocorticoid receptor from steroid-resistant proinflammatory CD8⁺ T cells
Other Titles: Bronchiolitis obliterans syndrome is associated with increased p-glycoprotein expression and loss of glucocorticoid receptor from steroid-resistant proinflammatory CD8(+) T cells
Author: Hodge, G.
Hodge, S.
Nguyen, P.
Yeo, A.
Sarkar, P.
Badiei, A.
Holmes-Liew, C.
Reynolds, P.
Holmes, M.
Citation: Clinical and Experimental Immunology, 2018; 192(2):242-250
Publisher: Wiley-Blackwell
Issue Date: 2018
ISSN: 0009-9104
1365-2249
Statement of
Responsibility: 
G. Hodge, S. Hodge, P. T. Nguyen, A. Yeo, P. Sarkar, A. Badiei, C. L. Holmes-Liew, P. N. Reynolds and M. Holmes
Abstract: Immunosuppressive therapy fails to suppress the production of proinflammatory cytokines, particularly by CD8+ T cells, in stable lung transplant recipients and those undergoing chronic rejection, suggesting that some patients may become relatively resistant to immunosuppressants such as glucocorticoids (GC). We have shown loss of GC receptor (GCR) from the CD8+ cells, and we hypothesized that the drug membrane efflux pump, p-glycoprotein-1 (Pgp), may also be involved in lymphocyte steroid resistance following lung transplant. Pgp/GCR expression and interferon (IFN)-γ/tumour necrosis factor (TNF)-α proinflammatory cytokine production was measured in blood lymphocytes from 15 stable lung transplant patients, 10 patients with bronchiolitis obliterans syndrome (BOS) and 10 healthy aged-matched controls (± prednisolone ± Pgp inhibitor, cyclosporin A ± GCR activator, Compound A) using flow cytometry. Both Pgp+ and Pgp- lymphocyte subsets from all subjects produced IFN-γ/TNF-α proinflammatory cytokines. Pgp expression was increased in CD8+ Pgp+ T cells and correlated with IFN-γ/TNF-α expression and BOS grade. Reduced GCR was observed in CD8+ Pgp- T, natural killer (NK) T-like and NK cells from stable patients compared with controls, and reduced further in CD8+ Pgp- T cells in BOS. The addition of 2·5 ng/ml cyclosporin A and 1 µM prednisolone inhibit IFN-γ/TNF-α production significantly by CD8+ Pgp+ T cells from BOS patients. The addition of 10 µM Compound A and 1 µM prednisolone inhibit IFN-γ/TNF-α production significantly by CD8+ Pgp- T cells from BOS patients. BOS is associated with increased Pgp expression and loss of GCR from steroid-resistant proinflammatory CD8+ T cells. Treatments that inhibit Pgp and up-regulate GCR in CD8+ T cells may improve graft survival.
Keywords: GCR; IFN-γ/TNF-α; lung transplant; Pgp; T cells
Rights: © 2018 British Society for Immunology
RMID: 0030084197
DOI: 10.1111/cei.13103
Appears in Collections:Medicine publications

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