Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/113205
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Type: Journal article
Title: Activity of bacteriophages in removing biofilms of pseudomonas aeruginosa isolates from chronic rhinosinusitis patients
Author: Fong, S.
Drilling, A.
Morales, S.
Cornet, M.
Woodworth, B.
Fokkens, W.
Psaltis, A.
Vreugde, S.
Wormald, P.
Citation: Frontiers in Cellular and Infection Microbiology, 2017; 7(SEP):1-11
Publisher: Frontiers
Issue Date: 2017
ISSN: 2235-2988
2235-2988
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Responsibility: 
Stephanie A. Fong, Amanda Drilling, Sandra Morales, Marjolein E. Cornet, Bradford A. Woodworth, Wytske J. Fokkens, Alkis J. Psaltis, Sarah Vreugde and Peter-John Wormald
Abstract: Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance. Biofilms grown in vitro were treated with a cocktail of four phages (CT-PA). Biofilm biomass was measured after 24 and 48 h, using a crystal violet assay. Phage titrations were performed to confirm replication of the phages. A linear mixed effects model was applied to assess the effects of treatment, time, CF status, and multidrug resistance on the biomass of the biofilm. Results: The isolates included 44 strain types. CT-PA treatment significantly reduced biofilm biomass at both 24 and 48 h post-treatment (p < 0.0001), regardless of CF status or antibiotic resistance. Biomass was decreased by a median of 76% at 48 h. Decrease in biofilm was accompanied by a rise in phage titres for all except one strain. Conclusion: A single dose of phages is able to significantly reduce biofilms formed in vitro by a range of P.aeruginosa isolates from CRS patients. This represents an exciting potential and novel targeted treatment for P. aeruginosa biofilm infections and multidrug resistant bacteria.
Keywords: Bacteriophage; Pseudomonas aeruginosa; chronic rhinosinusitis; cystic fibrosis; biofilm; multidrug resistant
Rights: © 2017 Fong, Drilling, Morales, Cornet, Woodworth, Fokkens, Psaltis, Vreugde and Wormald. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI: 10.3389/fcimb.2017.00418
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Microbiology and Immunology publications

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