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Type: Journal article
Title: ToF-SIMS multivariate analysis of surface-grafted small bioactive molecules
Author: Jasieniak, M.
Coad, B.
Griesser, H.
Citation: Biointerphases: an open access journal for the biomaterials interface community, 2015; 10(4):04A310-1-04A310-11
Publisher: American Institute of Physics
Issue Date: 2015
ISSN: 1934-8630
Statement of
Marek Jasieniak, Bryan R. Coad and Hans J. Griessera
Abstract: In the development of bioactive coatings on biomaterials, it is essential to characterize the successful fabrication and the uniformity of intended coatings by sensitive surface analytical techniques, so as to ensure reliable interpretation of observed biointerfacial responses. This can, however, be challenging when small bioactive molecules are grafted onto biomaterials surfaces at sub- and near-monolayer densities. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides the required sensitivity, but ion signals from small grafted molecules may still be dominated by fragment ions from the underlying polymer. In such cases, multivariate analysis provides valuable enhancement of spectral data, as illustrated here by examples comprising the surface grafting of bioactive serrulatane molecules, the peptide GRGDSP, the oligonucleotide 15-thymidine, and the antifungal compound Amphotericin B. The authors also show how ToF-SIMS plus principal component analysis can distinguish between covalent grafting and physisorption of the antibiotics caspofungin and micafungin.
Keywords: Biological Products
Coated Materials, Biocompatible
Spectrometry, Mass, Secondary Ion
Multivariate Analysis
Surface Properties
Rights: © 2015 American Vacuum Society.
DOI: 10.1116/1.4937464
Grant ID:
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Chemical Engineering publications

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