Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113416
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Structural characterisation of the HT3 motif of the polyhistidine triad protein D from Streptococcus pneumoniae.
Author: Luo, Z.
Pederick, V.G.
Paton, J.C.
McDevitt, C.A.
Kobe, B.
Citation: FEBS Letters, 2018; 592(13):2341-2350
Publisher: Wiley
Issue Date: 2018
ISSN: 0014-5793
1873-3468
Statement of
Responsibility: 
Zhenyao Luo, Victoria G. Pederick, James C. Paton, Christopher A. McDevitt and Bostjan Kobe
Abstract: The bacterium Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that requires Zn2+ for its survival and virulence in the host environment. Polyhistidine triad protein D (PhtD) has a known role in pneumococcal Zn2+ homeostasis. However, the mechanistic basis of PhtD function remains unclear, partly due to a lack of structural information. Here, we determined the crystal structure of the fragment PhtD269-339 , containing the third Zn2+ -binding histidine triad (HT) motif of the protein. Analysis of the structure suggests that Zn2+ -binding occurs at the surface of the protein and that all five HT motifs in the protein bind Zn2+ and share similar structures. These new structural insights aid in our understanding of how the Pht proteins facilitate pneumococcal Zn2+ acquisition. This article is protected by copyright. All rights reserved.
Keywords: In situ proteolysis; PhtD; Polyhistidine triad; Streptococcus pneumoniae; X-ray crystallography; Zinc acquisition
Rights: Copyright 2018 Federation of European Biochemical Societies
RMID: 0030090272
DOI: 10.1002/1873-3468.13122
Grant ID: http://purl.org/au-research/grants/arc/DP170102102
http://purl.org/au-research/grants/nhmrc/1071659
http://purl.org/au-research/grants/nhmrc/1080784
http://purl.org/au-research/grants/nhmrc/1122582
http://purl.org/au-research/grants/nhmrc/1110971
http://purl.org/au-research/grants/arc/FT170100006
Appears in Collections:Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.