Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/113522
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Type: Journal article
Title: Cell-type-specific H⁺-ATPase activity in root tissues enables K⁺ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress
Other Titles: Cell-type-specific H(+)-ATPase activity in root tissues enables K(+) retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress
Author: Shabala, L.
Zhang, J.
Pottosin, I.
Bose, J.
Zhu, M.
Fuglsang, A.
Velarde-Buendia, A.
Massart, A.
Hill, C.
Roessner, U.
Bacic, A.
Wu, H.
Azzarello, E.
Pandolfi, C.
Zhou, M.
Poschenrieder, C.
Mancuso, S.
Shabala, S.
Citation: Plant Physiology, 2016; 172(4):2445-2458
Publisher: American Society of Plant Biologists
Issue Date: 2016
ISSN: 0032-0889
1532-2548
Statement of
Responsibility: 
Lana Shabala, Jingyi Zhang, Igor Pottosin, Jayakumar Bose, Min Zhu, Anja Thoe Fuglsang, Ana Velarde-Buendia, Amandine Massart, Camilla Beate Hill, Ute Roessner, Antony Bacic, Honghong Wu, Elisa Azzarello, Camilla Pandolfi, Meixue Zhou, Charlotte Poschenrieder, Stefano Mancuso, and Sergey Shabala
Abstract: While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na+ uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na+, suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na+ exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K+ efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K+ retention ability are (1) an intrinsically lower H+-ATPase activity in the root apex, (2) greater salt-induced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2- to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.
Keywords: Hordeum
Description: Published October 21, 2016.
Rights: © 2016 American Society of Plant Biologists. All Rights Reserved.
DOI: 10.1104/pp.16.01347
Grant ID: http://purl.org/au-research/grants/arc/CE1101007
Appears in Collections:Agriculture, Food and Wine publications
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