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Type: Journal article
Title: Asexual female gametogenesis involves contact with a sexually fated megaspore in apomictic hieracium
Author: Juranić, M.
Tucker, M.
Schultz, C.
Shirley, N.
Taylor, J.
Spriggs, A.
Johnson, S.
Bulone, V.
Koltunow, A.
Citation: Plant Physiology, 2018; 177(3):1027-1049
Publisher: American Society of Plant Biologists
Issue Date: 2018
ISSN: 0032-0889
Statement of
Martina Juranić, Matthew R. Tucker, Carolyn J. Schultz, Neil J. Shirley, Jennifer M. Taylor, Andrew Spriggs, Susan D. Johnson, Vincent Bulone, and Anna M. Koltunow
Abstract: Apomixis results in asexual seed formation where progeny are identical to the maternal plant. In ovules of apomictic, Hieracium subgenus Pilosella species, which primarily form seeds asexually, the meiotic events of sexual female gametophyte (sFG) development occur, generating four megaspores. Aposporous initial (AI) cells form during meiosis in most ovules. The sexual pathway terminates during the events of functional megaspore (FM) differentiation when an enlarged AI undergoes mitosis to form an aposporous FG (aFG). The mitotically programmed FM dies along with the three other megaspores at this time by unknown mechanisms. Transcriptomes of laser-dissected AIs, ovule cells and ovaries from apomicts and AI-deficient mutants were analyzed to understand the pathways involved. The steps leading to AI mitosis and sexual pathway termination were determined using antibodies against arabinogalactan protein (AGP) epitopes found to mark both sexual and aFG lineages at inception. At most, four AIs differentiated near developing megaspores. The first expanding AI cell to contact the FM formed a functional AI (FAI) that underwent mitosis soon after megaspore degeneration. In silico analyses indicted that the enlarged, laser-captured AIs were arrested in the S/G2 phase of the cell cycle and were metabolically active. Further comparisons with AI-deficient mutants showed AIs were enriched in transcripts encoding homologs of genes involved in, and potentially antagonistic to, known FM specification pathways. We propose that AI and FM cell contact provides cues required for AI mitosis and megaspore degeneration. Specific candidates to further interrogate AI-FM interactions were identified here and include Hieracium AGP family genes.
Keywords: Plants, Genetically Modified
Plant Proteins
Gene Expression Profiling
Cell Cycle
Gene Expression Regulation, Plant
Genome, Plant
Carbohydrate Metabolism
Plant Cells
Rights: © 2018 American Society of Plant Biologists. All Rights Reserved.
DOI: 10.1104/pp.18.00342
Grant ID: ARC
Appears in Collections:Agriculture, Food and Wine publications
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