Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Full metadata record
DC FieldValueLanguage
dc.contributor.authorVoyle, R.-
dc.contributor.authorHaines, B.-
dc.contributor.authorLoffler, K.-
dc.contributor.authorHope, R.-
dc.contributor.authorRathjen, P.-
dc.contributor.authorBreed, W.-
dc.identifier.citationZygote: the biology of gametes and early embryos, 1999; 7(3):239-248-
dc.description.abstractThe zona pellucida (ZP) is an extracellular glycoprotein coat that is deposited around the oocyte during folliculogenesis and performs several functions that relate to fertilisation and preimplantion development. In eutherian mammals it consists of three major glycoproteins--ZPA, ZPB, and ZPC--but little is known about its molecular constitution in marsupials. We have isolated the cDNA encoding the ZPA homologue in two distantly related marsupial series: the possum, Trichosurus vulpecula (a phalangerid) and the dunnart Sminthopsis crassicaudata (a dasyurid). The two cDNA sequences were 86% identical and showed extensive regions of homology to eutherian ZPA proteins, particularly in the central region of the molecule. Many other features of the ZPA protein, except the positioning of the N-linked glycosylation sites, were also conserved between marsupials and eutherians. ZPA expression was shown to occur maximally in the cytoplasm of the oocyte primary follicles with a little, but significant, expression in oocytes of both primordial follicles and in the cytoplasm of the oocyte in follicles with an antral cavity. No expression was seen in surrounding follicle or granulosa cells.-
dc.rightsCopyright © 1999 Cambridge University Press-
dc.subjectOvarian Follicle-
dc.subjectZona Pellucida-
dc.subjectEgg Proteins-
dc.subjectMembrane Glycoproteins-
dc.subjectReceptors, Cell Surface-
dc.subjectIn Situ Hybridization-
dc.subjectReverse Transcriptase Polymerase Chain Reaction-
dc.subjectTranscription, Genetic-
dc.subjectAmino Acid Sequence-
dc.subjectBase Sequence-
dc.subjectSequence Homology, Amino Acid-
dc.subjectMolecular Sequence Data-
dc.subjectZona Pellucida Glycoproteins-
dc.titleIsolation and characterisation of zona pellucida A (ZPA) cDNAs from two species of marsupial: regulated oocyte-specific expression of ZPA transcripts-
dc.typeJournal article-
dc.identifier.orcidBreed, W. [0000-0003-1739-6593]-
Appears in Collections:Aurora harvest 2
Biochemistry publications
Environment Institute publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.