Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113777
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dc.contributor.authorWorthing, K.en
dc.contributor.authorPang, S.en
dc.contributor.authorTrott, D.en
dc.contributor.authorAbraham, S.en
dc.contributor.authorCoombs, G.en
dc.contributor.authorJordan, D.en
dc.contributor.authorMcIntyre, L.en
dc.contributor.authorDavies, M.en
dc.contributor.authorNorris, J.en
dc.date.issued2018en
dc.identifier.citationVeterinary Microbiology, 2018; 222:98-104en
dc.identifier.issn0378-1135en
dc.identifier.issn1873-2542en
dc.identifier.urihttp://hdl.handle.net/2440/113777-
dc.description.abstractThis study used phenotypic tests and whole genome sequencing to characterise a collection of 37 clinical Staphylococcus felis isolates from cats. Samples were isolated from a range of diseases including feline lower urinary tract disease (n = 15), otitis externa (n = 13), and ocular disease (n = 2). Isolates were identified using MALDI-TOF MS and by BLASTn analysis of S. felis-specific 16S rRNA, rpoB and nuc genes in whole genome sequence-based contigs. Phenotypic antimicrobial resistance was determined using disk diffusion and broth microdilution. Coagulase activity was assessed using feline and rabbit plasma. Genomes were screened for putative virulence and antimicrobial resistance genes using the sequences of known genes from other staphylococci as homologous references. Phylogenetic relationships were inferred using single nucleotide polymorphisms. One isolate was coagulase-positive when tested with feline plasma but all isolates were rabbit plasma coagulase-negative. No genetic determinant of coagulase activity was identified in this isolate. A range of putative virulence genes were found amongst isolates including genes associated with adhesion, toxin production and immune evasion. Ninety two percent of isolates were fully susceptible to all antimicrobials tested, which was reflected by a general absence of resistance genes. Clustering within the phylogenetic tree suggested a multiclonal population structure; this clustering did not correlate with disease syndrome or geographic origin of the isolate. Future studies of veterinary staphylococci will benefit from the publicly available S. felis draft genomes that were generated in this study.en
dc.description.statementofresponsibilityKate Worthing, Stanley Pang, Darren J. Trott, Sam Abraham, Geoffrey W Coombs, David Jordan, Liam McIntyre, Mark R Davies, Jacqueline Norrisen
dc.language.isoenen
dc.publisherElsevier BVen
dc.rights© 2018 Elsevier B.V. All rights reserved.en
dc.subjectStaphylococcus felis; feline medicine; Bacterial virulence; antimicrobial resistance; coagulase-Negative staphylococcien
dc.titleCharacterisation of Staphylococcus felis isolated from cats using whole genome sequencingen
dc.typeJournal articleen
dc.identifier.rmid0030095398en
dc.identifier.doi10.1016/j.vetmic.2018.07.002en
dc.relation.granthttp://purl.org/au-research/grants/arc/LP130100736en
dc.identifier.pubid432621-
pubs.library.collectionAnimal and Veterinary Sciences publicationsen
pubs.library.teamDS03en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Animal and Veterinary Sciences publications

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