Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113825
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Type: Journal article
Title: A locus on barley chromosome 5H affects adult plant resistance to powdery mildew
Author: Gupta, S.
Vassos, E.
Sznajder, B.
Fox, R.
Khoo, K.
Loughman, R.
Chalmers, K.
Mather, D.
Citation: Molecular Breeding, 2018; 38(8):103-1-103-10
Publisher: Springer
Issue Date: 2018
ISSN: 1380-3743
1572-9788
Statement of
Responsibility: 
Sanjiv Gupta, Elysia Vassos, Beata Sznajder, Rebecca Fox, Kelvin H. P. Khoo, Robert Loughman, Kenneth J. Chalmers, Diane E. Mather
Abstract: Adult plant resistance against plant pathogens is of interest as a means to achieve durable resistance. Prior to this research, the barley lines CLE210 (from Uruguay) and Denar (from the Czech Republic) had been reported to exhibit adult-plant resistance against powdery mildew. Here, populations of doubled haploid lines from crosses of these lines with the susceptible cultivar Baudin were evaluated for powdery mildew resistance in field experiments. Using linkage maps constructed from genotyping-by-sequencing (GBS) data, it was determined that differences in resistance were largely attributable to a region on the long arm of chromosome 5H (5HL). Therefore, KASP™ assays were developed based on GBS tag sequences mapped on that chromosome, providing more reliable genetic maps. In each population, a large-effect QTL was mapped on 5HL. As no sequence variation was detected between CLE210 and Denar in this region of 5HL, the two sources of resistance may be identical by descent in the QTL region and carry the same resistance gene. Marker assays from the QTL region were evaluated on a panel of barley lines, providing information that breeders could use to select assays for use in marker-assisted selection.
Keywords: Adult-plant resistance; quantitative trait loci; molecular markers; Blumeria graminis; Hordeum vulgare
Rights: © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
RMID: 0030095406
DOI: 10.1007/s11032-018-0858-2
Appears in Collections:Agriculture, Food and Wine publications

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