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Type: Journal article
Title: A fluorometric method for the quantification of cell number in complex differentiating osteoblast-osteocyte cultures
Author: Yang, D.
Wijenayaka, A.
Atkins, G.
Citation: Methods and Protocols, 2018; 1(2):14
Publisher: MDPI
Issue Date: 2018
ISSN: 2409-9279
Statement of
Dongqing Yang, Asiri R. Wijenayaka and Gerald J. Atkins
Abstract: Osteoblast/osteocyte cultures continue to emerge as essential tools for bone biology research in vitro. The change in cell number is an important parameter to be considered for investigating osteogenic differentiation. However, there is no reliable method for quantifying absolute cell count in differentiating osteoblast/osteocyte cultures because of their strongly adherent, multi-layered, super-confluent nature, and their accumulated extracellular matrix production which progressively mineralises in vitro. We developed a practical, simple and cost-effective method based on the fluorometric quantification of a nucleic dye, GelRed™, to enumerate cell number in osteoblast/osteocyte cultures. This method may also be suitable for quantifying cell numbers on other mammalian adherent cell types
Keywords: Osteoblast; osteocyte; GelRed™; mineralisation; cell number; fluorometric quantification
Rights: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
RMID: 0030090791
DOI: 10.3390/mps1020014
Appears in Collections:Medicine publications

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