Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/113921
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dc.contributor.authorYang, D.en
dc.contributor.authorWijenayaka, A.en
dc.contributor.authorAtkins, G.en
dc.date.issued2018en
dc.identifier.citationMethods and Protocols, 2018; 1(2):14en
dc.identifier.issn2409-9279en
dc.identifier.urihttp://hdl.handle.net/2440/113921-
dc.description.abstractOsteoblast/osteocyte cultures continue to emerge as essential tools for bone biology research in vitro. The change in cell number is an important parameter to be considered for investigating osteogenic differentiation. However, there is no reliable method for quantifying absolute cell count in differentiating osteoblast/osteocyte cultures because of their strongly adherent, multi-layered, super-confluent nature, and their accumulated extracellular matrix production which progressively mineralises in vitro. We developed a practical, simple and cost-effective method based on the fluorometric quantification of a nucleic dye, GelRed™, to enumerate cell number in osteoblast/osteocyte cultures. This method may also be suitable for quantifying cell numbers on other mammalian adherent cell typesen
dc.description.statementofresponsibilityDongqing Yang, Asiri R. Wijenayaka and Gerald J. Atkinsen
dc.language.isoenen
dc.publisherMDPIen
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectOsteoblast; osteocyte; GelRed™; mineralisation; cell number; fluorometric quantificationen
dc.titleA fluorometric method for the quantification of cell number in complex differentiating osteoblast-osteocyte culturesen
dc.typeJournal articleen
dc.identifier.rmid0030090791en
dc.identifier.doi10.3390/mps1020014en
dc.identifier.pubid425324-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS10en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidYang, D. [0000-0002-5471-6288]en
dc.identifier.orcidAtkins, G. [0000-0002-3123-9861]en
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