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Type: Journal article
Title: The molecular organisation of a B chromosome tandem repeat sequence from Brachycome dichromosomatica
Author: Franks, T.
Houben, A.
Leach, C.
Timmis, J.
Citation: Chromosoma: biology of the nucleus, 1996; 105(4):223-230
Issue Date: 1996
ISSN: 0009-5915
Statement of
Franks, T. ; Houben, A. ; Leach, C. ; Timmis, J.
Abstract: A high copy, tandemly repeated, sequence (Bd49) specific to the B chromosome and located near the centromere in Brachycome dichromosomatica was used to identify lambda genomic clones from DNA of a 3B plant. Only one clone of those analysed was composed entirely of a tandem array of the B-specific repeat unit. In other clones, the Bd49 repeats were linked to, or interspersed with, sequences that are repetitious and distributed elsewhere on the A and B chromosomes. One such repetitious flanking sequence has similarity to retrotransposon sequences and a second is similar to chloroplast DNA sequences. Of the four separate junctions analysed of Bd49-like sequence with flanking sequence, three were associated with the same A/T-rich region in Bd49 and the fourth was close to a 25 bp imperfect dyadic sequence. No novel B-specific sequences were detected within the genomic clones.
Keywords: Chromosomes
Deoxyribonucleases, Type II Site-Specific
DNA, Plant
Blotting, Southern
Cloning, Molecular
Polymerase Chain Reaction
Sequence Analysis, DNA
Base Sequence
Repetitive Sequences, Nucleic Acid
Sequence Homology, Nucleic Acid
Molecular Sequence Data
DOI: 10.1007/BF02528770
Appears in Collections:Aurora harvest 7
Genetics publications

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