Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11408
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Franks, T. | - |
dc.contributor.author | Houben, A. | - |
dc.contributor.author | Leach, C. | - |
dc.contributor.author | Timmis, J. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Chromosoma: biology of the nucleus, 1996; 105(4):223-230 | - |
dc.identifier.issn | 0009-5915 | - |
dc.identifier.issn | 1432-0886 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11408 | - |
dc.description.abstract | A high copy, tandemly repeated, sequence (Bd49) specific to the B chromosome and located near the centromere in Brachycome dichromosomatica was used to identify lambda genomic clones from DNA of a 3B plant. Only one clone of those analysed was composed entirely of a tandem array of the B-specific repeat unit. In other clones, the Bd49 repeats were linked to, or interspersed with, sequences that are repetitious and distributed elsewhere on the A and B chromosomes. One such repetitious flanking sequence has similarity to retrotransposon sequences and a second is similar to chloroplast DNA sequences. Of the four separate junctions analysed of Bd49-like sequence with flanking sequence, three were associated with the same A/T-rich region in Bd49 and the fourth was close to a 25 bp imperfect dyadic sequence. No novel B-specific sequences were detected within the genomic clones. | - |
dc.description.statementofresponsibility | Franks, T. ; Houben, A. ; Leach, C. ; Timmis, J. | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER VERLAG | - |
dc.source.uri | http://dx.doi.org/10.1007/bf02528770 | - |
dc.subject | Chromosomes | - |
dc.subject | Plants | - |
dc.subject | Deoxyribonucleases, Type II Site-Specific | - |
dc.subject | DNA, Plant | - |
dc.subject | Blotting, Southern | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Polymerase Chain Reaction | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Base Sequence | - |
dc.subject | Repetitive Sequences, Nucleic Acid | - |
dc.subject | Sequence Homology, Nucleic Acid | - |
dc.subject | Molecular Sequence Data | - |
dc.title | The molecular organisation of a B chromosome tandem repeat sequence from Brachycome dichromosomatica | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1007/BF02528770 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 7 Genetics publications |
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