Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Full metadata record
DC FieldValueLanguage
dc.contributor.authorOrth, A.-
dc.contributor.authorGhosh, R.-
dc.contributor.authorWilson, E.-
dc.contributor.authorDoughney, T.-
dc.contributor.authorBrown, H.-
dc.contributor.authorReineck, P.-
dc.contributor.authorThompson, J.-
dc.contributor.authorGibson, B.-
dc.identifier.citationBiomedical Optics Express, 2018; 9(7):2943-2954-
dc.description.abstractFluorescence microscopy is widely used to observe and quantify the inner workings of the cell. Traditionally, multiple types of cellular structures or biomolecules are visualized simultaneously in a sample by using spectrally distinct fluorescent labels. The wide emission spectra of most fluorophores limits spectral multiplexing to four or five labels in a standard fluorescence microscope. Further multiplexing requires another dimension of contrast. Here, we show that photostability differences can be used to distinguish between fluorescent labels. By combining photobleaching characteristics with a novel unmixing algorithm, we resolve up to three fluorescent labels in a single spectral channel and unmix fluorescent labels with nearly identical emission spectra. We apply our technique to organic dyes, autofluorescent biomolecules and fluorescent proteins. Our approach has the potential to triple the multiplexing capabilities of any digital widefield or confocal fluorescence microscope with no additional hardware, making it readily accessible to a wide range of researchers.-
dc.description.statementofresponsibilityAntony Orth, Richik N. Ghosh, Emma R. Wilson, Timothy Doughney, Hannah Brown, Philipp Reineck, Jeremy G. Thompson and Brant C. Gibson-
dc.publisherOSA Publishing-
dc.rights© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement-
dc.subject(110.4234) Multispectral and hyperspectral imaging-
dc.subject(170.2520) Fluorescence microscopy-
dc.titleSuper-multiplexed fluorescence microscopy via photostability contrast-
dc.typeJournal article-
dc.identifier.orcidDoughney, T. [0000-0003-0063-0048]-
dc.identifier.orcidBrown, H. [0000-0001-6342-3316]-
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]-
Appears in Collections:Aurora harvest 8
Medicine publications

Files in This Item:
File Description SizeFormat 
hdl_114092.pdfPublished version3.04 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.