Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/114103
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: A Novel Biodegradable Polyurethane Matrix for Auricular Cartilage Repair: An In Vitro and In Vivo Study
Author: Iyer, K.
Dearman, B.L.
Wagstaff, M.J.
Greenwood, J.E.
Citation: Journal of Burn Care and Research, 2016; 37(4):e353-e364
Publisher: Oxford University Press
Issue Date: 2016
ISSN: 1559-047X
1559-0488
Statement of
Responsibility: 
Kartik Iyer, Bronwyn L. Dearman, Marcus J. D. Wagstaff, John E. Greenwood
Abstract: Auricular reconstruction poses a challenge for reconstructive and burns surgeons. Techniques involving cartilage tissue engineering have shown potential in recent years. A biodegradable polyurethane matrix developed for dermal reconstruction offers an alternative to autologous, allogeneic, or xenogeneic biologicals for cartilage reconstruction. This study assesses such a polyurethane matrix for this indication in vivo and in vitro. To evaluate intrinsic cartilage repair, three pigs underwent auricular surgery to create excisional cartilage ± perichondrial defects, measuring 2 × 3 cm in each ear, into which acellular polyurethane matrices were implanted. Biopsies were taken at day 28 for histological assessment. Porcine chondrocytes ± perichondrocytes were cultured and seeded in vitro onto 1 × 1 cm polyurethane scaffolds. The total culture period was 42 days; confocal, histological, and immunohistochemical analyses of scaffold cultures were performed on days 14, 28, and 42. In vivo, the polyurethane matrices integrated with granulation tissue filling all biopsy samples. Minimal neocartilage invasion was observed marginally on some samples. Tissue composition was identical between ears whether perichondrium was left intact, or not. In vitro, the polyurethane matrix was biocompatible with chondrocytes ± perichondrocytes and supported production of extracellular matrix and Type II collagen. No difference was observed between chondrocyte culture alone and chondrocyte/perichondrocyte scaffold coculture. The polyurethane matrix successfully integrated into the auricular defect and was a suitable scaffold in vitro for cartilage tissue engineering, demonstrating its potential application in auricular reconstruction.
Keywords: Extracellular matrix; biopsy; burns; cartilage; chondrocytes; ear; ear cartilage; engineering; granulation tissue; polyurethanes; reconstructive surgical procedures; surgical procedures; operative; suidae; ear surgery; reconstructive; perichondrium
Rights: © 2015 by the American Burn Association
DOI: 10.1097/BCR.0000000000000281
Appears in Collections:Aurora harvest 3
Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.