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dc.contributor.authorLane, S.en
dc.contributor.authorJones, K.en
dc.identifier.citationJournal of Cell Biology, 2017; 216(12):3949-3957en
dc.description.abstractThe spindle assembly checkpoint (SAC) prevents chromosome missegregation by coupling anaphase onset with correct chromosome attachment and tension to microtubules. It does this by generating a diffusible signal from free kinetochores into the cytoplasm, inhibiting the anaphase-promoting complex (APC). The volume in which this signal remains effective is unknown. This raises the possibility that cell volume may be the reason the SAC is weak, and chromosome segregation error-prone, in mammalian oocytes. Here, by a process of serial bisection, we analyzed the influence of oocyte volume on the ability of the SAC to inhibit bivalent segregation in meiosis I. We were able to generate oocytes with cytoplasmic volumes reduced by 86% and observed changes in APC activity consistent with increased SAC control. However, bivalent biorientation remained uncoupled from APC activity, leading to error-prone chromosome segregation. We conclude that volume is one factor contributing to SAC weakness in oocytes. However, additional factors likely uncouple chromosome biorientation with APC activity.en
dc.description.statementofresponsibilitySimon I.R. Lane and Keith T. Jonesen
dc.publisherRockefeller University Pressen
dc.rights© 2017 Lane and Jones This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http ://www .rupress .org /terms /). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https ://creativecommons .org /licenses /by -nc -sa /4 .0 /).en
dc.subjectOocytes; Kinetochores; Microtubules; Animals; Mice, Inbred C57BL; Mice; Milrinone; Nocodazole; Gonadotropins, Equine; RNA, Complementary; Microinjections; Chromosome Segregation; Meiosis; Cell Size; Gene Expression Regulation; Female; Spindle Apparatus; Anaphase-Promoting Complex-Cyclosomeen
dc.titleChromosome biorientation and APC activity remain uncoupled in oocytes with reduced volumeen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
dc.identifier.orcidJones, K. [0000-0002-0294-0851]en
Appears in Collections:Molecular and Biomedical Science publications

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