Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/114177
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dc.contributor.authorLane, S.en
dc.contributor.authorJones, K.en
dc.date.issued2017en
dc.identifier.citationJournal of Cell Biology, 2017; 216(12):3949-3957en
dc.identifier.issn0021-9525en
dc.identifier.issn1540-8140en
dc.identifier.urihttp://hdl.handle.net/2440/114177-
dc.description.abstractThe spindle assembly checkpoint (SAC) prevents chromosome missegregation by coupling anaphase onset with correct chromosome attachment and tension to microtubules. It does this by generating a diffusible signal from free kinetochores into the cytoplasm, inhibiting the anaphase-promoting complex (APC). The volume in which this signal remains effective is unknown. This raises the possibility that cell volume may be the reason the SAC is weak, and chromosome segregation error-prone, in mammalian oocytes. Here, by a process of serial bisection, we analyzed the influence of oocyte volume on the ability of the SAC to inhibit bivalent segregation in meiosis I. We were able to generate oocytes with cytoplasmic volumes reduced by 86% and observed changes in APC activity consistent with increased SAC control. However, bivalent biorientation remained uncoupled from APC activity, leading to error-prone chromosome segregation. We conclude that volume is one factor contributing to SAC weakness in oocytes. However, additional factors likely uncouple chromosome biorientation with APC activity.en
dc.description.statementofresponsibilitySimon I.R. Lane and Keith T. Jonesen
dc.language.isoenen
dc.publisherRockefeller University Pressen
dc.rights© 2017 Lane and Jones This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http ://www .rupress .org /terms /). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https ://creativecommons .org /licenses /by -nc -sa /4 .0 /).en
dc.subjectOocytes; Kinetochores; Microtubules; Animals; Mice, Inbred C57BL; Mice; Milrinone; Nocodazole; Gonadotropins, Equine; RNA, Complementary; Microinjections; Chromosome Segregation; Meiosis; Cell Size; Gene Expression Regulation; Female; Spindle Apparatus; Anaphase-Promoting Complex-Cyclosomeen
dc.titleChromosome biorientation and APC activity remain uncoupled in oocytes with reduced volumeen
dc.typeJournal articleen
dc.identifier.rmid0030095270en
dc.identifier.doi10.1083/jcb.201606134en
dc.relation.granthttp://purl.org/au-research/grants/arc/DP120100946en
dc.identifier.pubid432967-
pubs.library.collectionMolecular and Biomedical Science publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidJones, K. [0000-0002-0294-0851]en
Appears in Collections:Molecular and Biomedical Science publications

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