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Type: Journal article
Title: Distinct modes of cyclin E/Cdc2c kinase regulation and S-phase control in mitotic and endoreduplicating cycles of Drosophila embryogenesis
Author: Sauer, Karsten
Knoblich, Jurgen A.
Richardson, Helena Elizabeth
Lehner, Christian F.
Citation: Genes and Development, 1995; 9(11):1327-1339
Publisher: Cold Spring Harbor Laboratory Press
Issue Date: 1995
ISSN: 0890-9369
Statement of
Karsten Sauer, Jürgen A. Knoblich, Helena Richardson, and Christian F. Lehner
Abstract: Drosophila cyclin E (DmcycE) is required in embryos for S phase of mitotic and endoreduplication cycles. Here, we describe regulatory differences characteristic for these two cell cycle types. While DmcycE transcript levels decline in DmcycE mutant cells programmed for mitotic proliferation, they are maintained and no longer restricted to transient pulses in DmcycE mutant cells programmed for endoreduplication. Moreover, DmcycE expression in endoreduplicating cells is down-regulated by ectopic expression of a heat-inducible cyclin E transgene. DmcycE expression in endoreduplicating tissues, therefore, is restricted by a negative feedback to the transient pulse triggering entry into S-phase. Conversely, during mitotic cycles, where S phase entry is not only dependent on cyclin E but also on progression through M phase, cyclin E and associated Dmcdc2c kinase activity are present throughout the cell cycle. Reinitiation of DNA replication during the G2 phase of the mitotic cell cycle, therefore, is prevented by cyclin E/Dmcdc2c kinase-independent regulation. Observations in cyclin A mutants implicate G2 cyclins in this regulation. Our results suggest molecular explanations for the different rules governing S phase during mitotic and endoreduplication cycles.
Keywords: Cyclin A; cyclin E; Dmcdc2c kinase; S phase; endoreduplication; Drosophila
Rights: © 1995 by Cold Spring Harbor Laboratory Press
DOI: 10.1101/gad.9.11.1327
Appears in Collections:Genetics publications

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