Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Exploring the roles of CREBRF and TRIM2 in the regulation of angiogenesis by high-density lipoproteins
Author: Wong, N.
Cheung, H.
Solly, E.
Vanags, L.
Ritchie, W.
Nicholls, S.
Ng, M.
Bursill, C.
Tan, J.
Citation: International Journal of Molecular Sciences, 2018; 19(7):1903-1-1903-17
Publisher: MDPI AG
Issue Date: 2018
ISSN: 1661-6596
Statement of
Nathan K.P. Wong, Helena Cheung, Emma L. Solly, Laura Z. Vanags, William Ritchie, Stephen J. Nicholls, Martin K.C. Ng, Christina A. Bursill, and Joanne T.M. Tan
Abstract: Angiogenesis, the process of forming new blood vessels, is crucial in the physiological response to ischemia, though it can be detrimental as part of inflammation and tumorigenesis. We have previously shown that high-density lipoproteins (HDL) modulate angiogenesis in a context-specific manner via distinct classical signalling pathways, enhancing hypoxia-induced angiogenesis while suppressing inflammatory-driven angiogenesis. Whether additional novel targets exist to account for these effects are unknown. A microarray approach identified two novel genes, cyclic-adenosine-monophosphate-response-element-binding protein 3 regulatory factor (CREBRF) and tripartite motif-containing protein 2 (TRIM2) that were upregulated by reconstituted HDL (rHDL). We measured CREBRF and TRIM2 expression in human coronary artery endothelial cells following incubation with rHDL and exposure to either hypoxia or an inflammatory stimulus. We found that CREBRF and TRIM2 mRNA were significantly upregulated by rHDL, particularly in response to its phospholipid component 1-palmitoyl-2-linoleoyl-phosphatidylcholine, however, protein expression was not significantly altered. Knockdown of TRIM2 impaired endothelial cell tubulogenesis in vitro in both hypoxia and inflammation, implying a necessary role in angiogenesis. Furthermore, TRIM2 knockdown attenuated rHDL-induced tubule formation in hypoxia, suggesting that it is important in mediating the pro-angiogenic action of rHDL. Our study has implications for understanding the regulation of angiogenesis in both of these pathophysiological contexts by HDL.
Keywords: Angiogenesis; high-density lipoproteins; hypoxia; inflammation; TRIM2; CREBRF
Rights: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
RMID: 0030094388
DOI: 10.3390/ijms19071903
Grant ID:
Appears in Collections:Medicine publications

Files in This Item:
File Description SizeFormat 
hdl_114358.pdfPublished Version23.26 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.