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Type: Journal article
Title: High-resolution mapping of crossover events in the hexaploid wheat genome suggests a universal recombination mechanism
Author: Darrier, B.
Rimbert, H.
Balfourier, F.
Pingault, L.
Josselin, A.
Servin, B.
Navarro, J.
Choulet, F.
Paux, E.
Sourdille, P.
Citation: Genetics: a periodical record of investigations bearing on heredity and variation, 2017; 206(3):1373-1388
Publisher: Genetics Society of America
Issue Date: 2017
ISSN: 0016-6731
Statement of
Benoit Darrier, Hélène Rimbert, François Balfourier, Lise Pingault, Ambre-Aurore Josselin, Bertrand Servin, Julien Navarro, Frédéric Choulet, Etienne Paux, and Pierre Sourdille
Abstract: During meiosis, crossovers (COs) create new allele associations by reciprocal exchange of DNA. In bread wheat (Triticum aestivum L.), COs are mostly limited to subtelomeric regions of chromosomes, resulting in a substantial loss of breeding efficiency in the proximal regions, though these regions carry ∼60-70% of the genes. Identifying sequence and/or chromosome features affecting recombination occurrence is thus relevant to improve and drive recombination. Using the recent release of a reference sequence of chromosome 3B and of the draft assemblies of the 20 other wheat chromosomes, we performed fine-scale mapping of COs and revealed that 82% of COs located in the distal ends of chromosome 3B representing 19% of the chromosome length. We used 774 SNPs to genotype 180 varieties representative of the Asian and European genetic pools and a segregating population of 1270 F6 lines. We observed a common location for ancestral COs (predicted through linkage disequilibrium) and the COs derived from the segregating population. We delineated 73 small intervals (<26 kb) on chromosome 3B that contained 252 COs. We observed a significant association of COs with genic features (73 and 54% in recombinant and nonrecombinant intervals, respectively) and with those expressed during meiosis (67% in recombinant intervals and 48% in nonrecombinant intervals). Moreover, while the recombinant intervals contained similar amounts of retrotransposons and DNA transposons (42 and 53%), nonrecombinant intervals had a higher level of retrotransposons (63%) and lower levels of DNA transposons (28%). Consistent with this, we observed a higher frequency of a DNA motif specific to the TIR-Mariner DNA transposon in recombinant intervals.
Keywords: Recombination; meiosis; bread wheat; linkage disequilibrium; transposon; hotspot; sequence motif
Rights: Copyright © 2017 by the Genetics Society of America
DOI: 10.1534/genetics.116.196014
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Appears in Collections:Agriculture, Food and Wine publications
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