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dc.contributor.authorRout-Pitt, N.en
dc.contributor.authorMcCarron, A.en
dc.contributor.authorMcIntyre, C.en
dc.contributor.authorParsons, D.en
dc.contributor.authorDonnelley, M.en
dc.identifier.citationJournal of Biological Methods, 2018; 5(2):e90-1-e90-9en
dc.description.abstractLentiviral-mediated gene therapy has been proposed for the treatment of a range of diseases, and due to its genome integration properties, it offers the potential for long-lasting benefit from a once-off treatment. Production methods for pre-clinical studies in animal models, and ultimately for human clinical trials, must be capable of producing large quantities of high-quality lentiviral vector in an efficient and cost-effective manner. We report here a medium-scale method (from 1.5 L to 6 L of vector supernatant) for lentiviral vector production in adherent cell cultures using the NUNC™ EasyFill™ Cell Factory™ from Thermo Fisher Scientific. Downstream purification uses a Mustang Q XT5 anion exchange capsule from Pall, and an ultracentrifugation step to concentrate the vector. This method is capable of producing lentiviral vector with concentrated titres of 108–109 TU/ml, with reduced manual handling compared to single monolayer flask methods.en
dc.description.statementofresponsibilityNathan Rout-Pitt, Alexandra McCarron, Chantelle McIntyre, David Parsons, Martin Donnelleyen
dc.publisherPOL Scientificen
dc.rights© The Authors. This work is licensed under a Creative Commons Attribution 3.0 License.en
dc.subjectCalcium phosphate; cell factories; lentiviral vector; Mustang Q; ultracentrifugationen
dc.titleLarge-scale production of lentiviral vectors using multilayer cell factoriesen
dc.typeJournal articleen
pubs.library.collectionMedicine publicationsen
dc.identifier.orcidRout-Pitt, N. [0000-0002-4003-4454]en
dc.identifier.orcidParsons, D. [0000-0003-1746-3290]en
dc.identifier.orcidDonnelley, M. [0000-0002-5320-7756]en
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Medicine publications

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