Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/114573
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dc.contributor.author | Rout-Pitt, N.B. | - |
dc.contributor.author | McCarron, A. | - |
dc.contributor.author | McIntyre, C. | - |
dc.contributor.author | Parsons, D. | - |
dc.contributor.author | Donnelley, M. | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Journal of Biological Methods, 2018; 5(2):e90-1-e90-9 | - |
dc.identifier.issn | 2326-9901 | - |
dc.identifier.issn | 2326-9901 | - |
dc.identifier.uri | http://hdl.handle.net/2440/114573 | - |
dc.description.abstract | Lentiviral-mediated gene therapy has been proposed for the treatment of a range of diseases, and due to its genome integration properties, it offers the potential for long-lasting benefit from a once-off treatment. Production methods for pre-clinical studies in animal models, and ultimately for human clinical trials, must be capable of producing large quantities of high-quality lentiviral vector in an efficient and cost-effective manner. We report here a medium-scale method (from 1.5 L to 6 L of vector supernatant) for lentiviral vector production in adherent cell cultures using the NUNC™ EasyFill™ Cell Factory™ from Thermo Fisher Scientific. Downstream purification uses a Mustang Q XT5 anion exchange capsule from Pall, and an ultracentrifugation step to concentrate the vector. This method is capable of producing lentiviral vector with concentrated titres of 108–109 TU/ml, with reduced manual handling compared to single monolayer flask methods. | - |
dc.description.statementofresponsibility | Nathan Rout-Pitt, Alexandra McCarron, Chantelle McIntyre, David Parsons, Martin Donnelley | - |
dc.language.iso | en | - |
dc.publisher | POL Scientific | - |
dc.rights | © The Authors. This work is licensed under a Creative Commons Attribution 3.0 License. | - |
dc.source.uri | http://dx.doi.org/10.14440/jbm.2018.236 | - |
dc.subject | Calcium phosphate; cell factories; lentiviral vector; Mustang Q; ultracentrifugation | - |
dc.title | Large-scale production of lentiviral vectors using multilayer cell factories | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.14440/jbm.2018.236 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1098127 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Rout-Pitt, N.B. [0000-0002-4003-4454] | - |
dc.identifier.orcid | Parsons, D. [0000-0003-1746-3290] | - |
dc.identifier.orcid | Donnelley, M. [0000-0002-5320-7756] | - |
Appears in Collections: | Aurora harvest 8 IPAS publications Medicine publications |
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hdl_114573.pdf | Published Version | 1.31 MB | Adobe PDF | View/Open |
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