Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/114937
Type: Theses
Title: Hypoxia-inducible factor-1α mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia
Author: Lang, Kenneth J. D.
Issue Date: 2003
School/Discipline: Dept. of Medicine
Abstract: HIF-1α is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1α protein is rapidly degraded in cells supplied with adequate oxygen, but is stabilised in hypoxic cells. Using polysome profile analysis, it was found that translation of HIF-1α mRNA in NIH3T3 cells is spared the general reduction in translation rate that occurs during hypoxia. Further investigation found that the HIF-1α 5'UTR promoted translation of the downstream reporter, indicating the presence of an internal ribosome entry site (IRES). Indications are that the presence of the IRES in the HIF-1α 5'UTR is to allow translation to be maintained under conditions that are inhibitory to cap-dependent translation.
Dissertation Note: Thesis (Ph.D.) -- University of Adelaide, Dept. of Medicine, 2003.
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals
Appears in Collections:Research Theses

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