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Type: Journal article
Title: Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae infection: Adelaide, 1978–1992
Author: Marmion, B.P.
Williamson, J.
Worswick, D.A.
Kok, T.W.
Harris, R.J.
Citation: Clinical Infectious Diseases, 1993; 17(Suppl. 1):S90-S99
Publisher: University of Chicago Press
Issue Date: 1993
ISSN: 1058-4838
Statement of
B. P. Marmion, J. Williamson, D. A. Worswick, T.-W. Kok, and R. J. Harris
Abstract: Efforts to improve laboratory diagnostic methods for infection due to Mycoplasma pneumoniae have involved the use of a cell-sheet culture method and a modified indirect hemagglutination method for IgM antibody, while direct detection of mycoplasma has employed antigen capture-enzyme immunoassay (Ag-EIA) and polymerase chain reaction (PCR) amplification of sequences within the P1 and 16S ribosomal RNA genes and quantification of the amplified DNA by dot blot hybridization (DBH). Cell-sheet culture was slightly more sensitive and more rapid than culture with cell-free diphasic medium. Indirect hemagglutination detection of IgM antibody to M. pneumoniae was more sensitive than CF and EIA for detection of IgM antibody to mycoplasma. Ag-EIA gave a rapid and reasonably sensitive indication of infection and correlated well with a serological response of patients indicating a current infection. PCR-DBH was a highly sensitive substitute for culture of mycoplasma. Both Ag-EIA and PCR-DBH require confirmation by assessment of serological response to verify that the infection is current and that positive results of PCR-DBH, in particular, are not the result of continuing carriage of the organism from a previous infection, unrelated to the current episode under investigation.
Keywords: Mycoplasma pneumoniae
Pneumonia, Mycoplasma
Antigens, Bacterial
Rights: © 1993 by The University of Chicago. All rights reserved.
DOI: 10.1093/clinids/17.Supplement_1.S90
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