Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/114950
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWilliamson, J.en
dc.contributor.authorMarmion, B.en
dc.contributor.authorWorswick, D.en
dc.contributor.authorKok, T.en
dc.contributor.authorTannock, G.en
dc.contributor.authorHerd, R.en
dc.contributor.authorHarris, R.en
dc.date.issued1992en
dc.identifier.citationEpidemiology and Infection, 1992; 109(3):519-537en
dc.identifier.issn0950-2688en
dc.identifier.issn1469-4409en
dc.identifier.urihttp://hdl.handle.net/2440/114950-
dc.description.abstractDirect detection assays for Mycoplasma pneumoniae were established by PCR amplification of short sequences within the foot protein/adhesin (P1) gene and the 16S ribosomal RNA gene. Specificity and sensitivity was excellent, no hybridization was observed with M. genitalium and other human Mycoplasma species. In nose and throat washings from subjects with respiratory infection a pattern of high counts (c.f.u./ml) of M. pneumoniae (deduced from the amount of amplified PCR product), and a positive antigen capture assay, was found in 83% of subjects with serological evidence of current infection with M. pneumoniae. A small proportion of subjects with serological patterns suggesting infection in the more distant past had positive PCR assays. This was considered to represent either persistence of the organism from a previous infection or perhaps transient carriage during a reinfection, without substantial change in antibody response. PCR-based assay of M. pneumoniae offers a powerful, rapid, and sensitive substitute for culture of the mycoplasma. Antigen capture, while less sensitive than PCR, offers the advantage that it is more often positive with samples from current infection and requires less stringent laboratory organization to contain false positive results. We conclude however that the laboratory diagnosis of a chosen clinical episode should not rest on the PCR or Ag-EIA assays alone, but must also include antibody assays to confirm whether infection is current or represents persistence from past exposure.en
dc.description.statementofresponsibilityJ. Williamson, B. P. Marmion, D. A. Worswick, T.-W. Kok, G. Tannock, R. Herd, and R. J. Harrisen
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.rights© Cambridge University Press 1992en
dc.subjectMycoplasma pneumoniae; RNA, Bacterial; RNA, Ribosomal, 16Sen
dc.titleLaboratory diagnosis of Mycoplasma pneumoniae infection. 4. Antigen capture and PCR-gene amplification for detection of the mycoplasma: problems of clinical correlationen
dc.typeJournal articleen
dc.identifier.rmid0030075260en
dc.identifier.doi10.1017/S0950268800050512en
dc.identifier.pubid369243-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS06en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.