Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press|
|Citation:||Biotechnology Letters, 1995; 9(7):477-480|
|Organisation:||Cooperative Research Centre for Tissue Growth and Repair|
|Lene Jorgensen, Brian K. O'Neill, Connor J. Thomas, Renato Morona and Anton P. J. Middelberg|
|Abstract:||The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris.|
|Appears in Collections:||Aurora harvest 7|
Microbiology and Immunology publications
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.