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Type: Journal article
Title: Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press
Author: Jorgensen, L.
O'Neill, B.
Thomas, C.
Morona, R.
Middelberg, A.
Citation: Biotechnology Letters, 1995; 9(7):477-480
Publisher: SPRINGER
Issue Date: 1995
ISSN: 0951-208X
Organisation: Cooperative Research Centre for Tissue Growth and Repair
Statement of
Lene Jorgensen, Brian K. O'Neill, Connor J. Thomas, Renato Morona and Anton P. J. Middelberg
Abstract: The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris.
DOI: 10.1007/BF00159561
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Appears in Collections:Aurora harvest 7
Microbiology and Immunology publications

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