Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/11539
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Type: Journal article
Title: Neutrophils activated by granulocyte-macrophage colony-stimulating factor express receptors for interleukin-3 which mediate class II expression
Author: Smith, W.
Guida, L.
Qiuy, S.
Korpelainen, E.
van den Hueven, C.
Gillis, D.
Hawrylowicz, C.
Vadas, M.
Lopez, A.
Citation: Blood, 1995; 86(10):3938-3944
Publisher: American Society of Hematology
Issue Date: 1995
ISSN: 0006-4971
1528-0020
Statement of
Responsibility: 
WB Smith, L Guida, Q Sun, EI Korpelainen, C van den Heuvel, D Gillis, CM Hawrylowicz, MA Vadas, and AF Lopez
Abstract: Freshly isolated peripheral blood neutrophils, unlike monocytes and eosinophils, do not bind interleukin-3 (IL-3) or respond to IL-3). We show that neutrophils cultured for 24 hours in granulocyte-macrophage colony-stimulating factor (GM-CSF) express mRNA for the IL-3 receptor (R) alpha subunit, as shown by RNase protection assays, and IL-3R alpha chain protein, as shown by cytometric analysis using two different specific monoclonal antibodies. This effect was selective for GM-CSF, because granulocyte colony-stimulating factor, tumor necrosis factor- alpha, interferon-gamma, and IL-1 failed to induce the IL-3 receptor. Saturation binding curves with 125I-IL-3 and Scatchard transformation showed the presence of about 100 high-affinity and 4,000 low-affinity receptors. Because neutrophils have been shown to express human leukocyte antigen (HLA)-DR in response to GM-CSF, we examined the possibility that IL-3 could augment HLA-DR expression on GM-CSF-treated cells. We found that neutrophils incubated with 30 ng/mL IL-3 as well as 0.1 ng/mL GM-CSF expressed a mean of 2.1-fold higher levels of HLA- DR than with GM-CSF alone (P < .005), confirming the signaling competence of the newly expressed IL-3R. This increase was seen even at maximal concentrations of GM-CSF and IL-3 can have an additive effect on mature human cells. The augmentation of HLA-DR by IL-3 was specific because it could be inhibited by a blocking anti-IL-3R antibody. Expression of class II molecules by neutrophils under these conditions may have significance for antigen presentation. These results provide further evidence for the role of GM-CSF as an amplification factor in inflammation by inducing neutrophil responsiveness to IL-3 produced by T cells or mast cells.
Keywords: Neutrophils; Cells, Cultured; Humans; Granulocyte-Macrophage Colony-Stimulating Factor; Interleukin-3; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; Receptors, Interleukin-3; Recombinant Proteins; RNA, Messenger; HLA-D Antigens; Flow Cytometry; Signal Transduction; Neutrophil Activation; Gene Expression Regulation
Rights: © 1995 by The American Society of Hematology
RMID: 0030004250
DOI: 10.1182/blood.v86.10.3938.bloodjournal86103938
Published version: http://www.bloodjournal.org/content/86/10/3938
Appears in Collections:Microbiology and Immunology publications

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