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Type: Journal article
Title: A patient-derived explant (PDE) model of hormone-dependent cancer
Author: Centenera, M.M.
Hickey, T.E.
Jindal, S.
Ryan, N.K.
Ravindranathan, P.
Mohammed, H.
Robinson, J.L.
Schiewer, M.J.
Ma, S.
Kapur, P.
Sutherland, P.D.
Hoffmann, C.E.
Roehrborn, C.G.
Gomella, L.G.
Carroll, J.S.
Birrell, S.N.
Knudsen, K.E.
Raj, G.V.
Butler, L.M.
Tilley, W.D.
Citation: Molecular Oncology, 2018; 12(9):1608-1622
Publisher: Wiley
Issue Date: 2018
ISSN: 1574-7891
Statement of
Margaret M. Centenera, Theresa E. Hickey, Shalini Jindal, Natalie K. Ryan, Preethi Ravindranathan, Hisham Mohammed, Jessica L. Robinson, Matthew J. Schiewer, Shihong Ma, Payal Kapur, Peter D. Sutherland, Clive E. Hoffmann, Claus G. Roehrborn, Leonard G. Gomella, Jason S. Carroll, Stephen N. Birrell, Karen E. Knudsen, Ganesh V. Raj, Lisa M. Butler, Wayne D. Tilley
Abstract: Breast and prostate cancer research to date has largely been predicated on the use of cell lines in vitro or in vivo. These limitations have led to the development of more clinically relevant models, such as organoids or murine xenografts that utilize patient-derived material; however, issues related to low take rate, long duration of establishment, and the associated costs constrain use of these models. This study demonstrates that ex vivo culture of freshly resected breast and prostate tumor specimens obtained from surgery, termed patient-derived explants (PDEs), provides a high-throughput and cost-effective model that retains the native tissue architecture, microenvironment, cell viability, and key oncogenic drivers. The PDE model provides a unique approach for direct evaluation of drug responses on an individual patient's tumor, which is amenable to analysis using contemporary genomic technologies. The ability to rapidly evaluate drug efficacy in patient-derived material has high potential to facilitate implementation of personalized medicine approaches.
Keywords: ex vivo culture
patient-derived explant
preclinical tumor model
Rights: © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1002/1878-0261.12354
Grant ID:
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