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|Title:||Contrasting roles for RANTES and macrophage inflammatory protein-1α (MIP-1α) in a murine model of allergic peritonitis|
|Other Titles:||Contrasting roles for RANTES and macrophage inflammatory protein-1 alpha (MIP-1 alpha) in a murine model of allergic peritonitis|
|Citation:||Clinical and Experimental Immunology, 1999; 117(2):223-229|
|Abstract:||Cell accumulation and CC chemokine production were assessed in the peritoneal cavity of ovalbumin (OVA)-sensitized mice following antigen challenge. Intraperitoneal challenge with OVA induced a significant eosinophil influx from 6 h post-challenge with increased numbers persisting at 24 h. At 6 h there was also a marked presence of neutrophils. Messenger RNA expression and protein levels for the chemokines RANTES and MIP-1 alpha were measured in the cell pellets and supernatants, respectively, from peritoneal washes following OVA challenge. RANTES mRNA was detected from 2 h to 4 h following OVA injection, whereas mRNA for MIP-1 alpha was only detectable at 4 h. RANTES protein was first detected from 4 h after OVA injection and by 24 h the protein levels had increased further. Basal levels of MIP-1 alpha were detected in peritoneal washes. These levels peaked at 2 h after OVA challenge and rapidly declined to basal levels by 6 h. A functional role for the chemokines was assessed using neutralizing polyclonal antibodies. Co-injection of OVA with anti-RANTES antibodies resulted in a significant inhibition of eosinophil infiltration into the cavity at 6 h and 24 h (63% and 52% inhibition, respectively) without significantly influencing the number of neutrophils present. In contrast, injection of anti-MIP-1 alpha antibodies only inhibited neutrophil migration at the 6 h time point by 44% without significantly affecting the accumulation of eosinophils. These results demonstrate an important role for RANTES in mediating eosinophil influx in allergic inflammation and a contrasting role for MIP-1 alpha in mediating neutrophil recruitment.|
|Keywords:||Peritoneal Cavity; Eosinophils; Neutrophils; Animals; Mice, Inbred BALB C; Mice; Peritonitis; Hypersensitivity; Disease Models, Animal; Ovalbumin; Macrophage Inflammatory Proteins; Immune Sera; Injections, Intraperitoneal; Cell Movement; Time Factors; Female; Chemokine CCL5; Chemokine CCL4|
|Appears in Collections:||Microbiology and Immunology publications|
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