Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/115554
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Type: Journal article
Title: Diversity of arsenite oxidase gene and arsenotrophic bacteria in arsenic affected Bangladesh soils
Author: Sanyal, S.
Mou, T.
Chakrabarty, R.
Hoque, S.
Hossain, M.
Sultana, M.
Citation: AMB Express, 2016; 6(1):21-1-21-11
Publisher: Springer
Issue Date: 2016
ISSN: 2191-0855
2191-0855
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Santonu Kumar Sanyal, Taslin Jahan Mou, Ram Prosad Chakrabarty, Sirajul Hoque, M. Anwar Hossain and Munawar Sultana
Abstract: Arsenic (As) contaminated soils are enriched with arsenotrophic bacteria. The present study analyzes the microbiome and arsenotrophic genes-from As affected soil samples of Bhanga, Charvadrason and Sadarpur of Faridpur district in Bangladesh in summer (SFDSL1, 2, 3) and in winter (WFDSL1, 2, 3). Total As content of the soils was within the range of 3.24–17.8 mg/kg as per atomic absorption spectroscopy. The aioA gene, conferring arsenite [As (III)] oxidation, was retrieved from the soil sample, WFDSL-2, reported with As concentration of 4.9 mg/kg. Phylogenetic analysis revealed that the aioA genes of soil WFDSL-2 were distributed among four major phylogenetic lineages comprised of α, β, γ Proteobacteria and Archaea with a dominance of β Proteobacteria (56.67 %). An attempt to enrich As (III) metabolizing bacteria resulted 53 isolates. ARDRA (amplified ribosomal DNA restriction analysis) followed by 16S rRNA gene sequencing of the 53 soil isolates revealed that they belong to six genera; Pseudomonas spp., Bacillus spp., Brevibacillus spp., Delftia spp., Wohlfahrtiimonas spp. and Dietzia spp. From five different genera, isolates Delftia sp. A2i, Pseudomonas sp. A3i, W. chitiniclastica H3f, Dietzia sp. H2f, Bacillus sp. H2k contained arsB gene and showed arsenite tolerance up-to 27 mM. Phenotypic As (III) oxidation potential was also confirmed with the isolates of each genus and isolate Brevibacillus sp. A1a showed significant As (III) transforming potential of 0.2425 mM per hour. The genetic information of bacterial arsenotrophy and arsenite oxidation added scientific information about the possible bioremediation potential of the soil isolates in Bangladesh.
Keywords: As metabolizing bacteria; arsenite oxidase gene (aioA); arsenite resistant; Bangladesh
Rights: © 2016 Sanyal et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
RMID: 0030053961
DOI: 10.1186/s13568-016-0193-0
Appears in Collections:Microbiology and Immunology publications

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