Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11561
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Type: Journal article
Title: Elevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosis
Author: Akoum, A.
Lemay, A.
McColl, S.
Turcot-Tremblay, L.
Maheux, R.
Citation: Fertility and Sterility, 1996; 66(1):17-23
Publisher: Elsevier BV
Issue Date: 1996
ISSN: 0015-0282
1556-5653
Abstract: <h4>Objective</h4>To estimate the concentration and the biologic activity of monocyte chemotactic protein-1 (MCP-1) in the peritoneal fluid (PF) of women with and without endometriosis.<h4>Design</h4>A case control study was conducted.<h4>Setting</h4>Gynecology clinic and Laboratories of endocrinology of reproduction and immunology.<h4>Patients</h4>Women presenting for infertility, pelvic pain, or tubal ligation in which endometriosis was diagnosed at laparoscopy (n = 36) and normal fertile controls presenting for tubal ligation (n = 21).<h4>Interventions</h4>Collection of PF via laparoscopy.<h4>Main outcome measures</h4>Determination of PF concentrations of MCP-1 by an ELISA and evaluation of its monocyte chemotactic activity using a human hystiocytic cell line (U937). RESULTS. The concentration of MCP-1 (median, range of values) was increased in the PF of endometriosis patients (283, 0 to 1,930 pg/mL; conversion factor to SI unit, 0.155) compared with the control group (140, 0 to 435 pg/mL). The most significant elevation of MCP-1 levels was found in the stage II of the disease (371, 200 to 1,930 pg/mL). An increased chemotactic activity for monocytes (mean number of migrating cells/mm2 +/- SD) also was found in stages I (1,460 +/- 312) and II (1,541 +/- 336) of the disease when compared with fertile controls (393 +/- 56). Forty percent to 53% of this activity was inhibited in the presence of an antibody specific to MCP-1.<h4>Conclusions</h4>These observations are consistent with previous data indicating increased leukocyte chemotaxis in the PF of patients with endometriosis and suggest that MCP-1 may play a relevant role in the peritoneal inflammatory reaction associated with the disease.
Keywords: Monocytes
Ascitic Fluid
Humans
Endometriosis
Case-Control Studies
Chemotaxis, Leukocyte
Osmolar Concentration
Adult
Female
Chemokine CCL2
DOI: 10.1016/s0015-0282(16)58381-2
Appears in Collections:Aurora harvest 2
Microbiology and Immunology publications

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