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Type: Journal article
Title: Dependance of epithelial growth of the small intestine on T-cell activation during weaning in the rat
Author: Thompson, F.
Mayrhofer, G.
Cummins, A.
Citation: Gastroenterology, 1996; 111(1):37-44
Publisher: W B SAUNDERS CO
Issue Date: 1996
ISSN: 0016-5085
Abstract: <h4>Background & aims</h4>Intestinal crypt hyperplasia is associated with local T-cell activation in both clinical and experimental examples of immunologically mediated enteropathy. This suggests that T cell-derived factors may be trophic for epithelial proliferation in the intestine postnatally. The purpose of this study was to investigate T-cell activity during weaning in the rat and to investigate immune dependence of intestinal growth on T-cell activation.<h4>Methods</h4>The expression of interleukin-2 receptor (IL-2R) by mesenteric lymph node T cells was investigated from days 14 to 160 of life. Rats were treated with monoclonal antibodies against the IL-2R that were nonblocking (control) or blocking (experimental) from day 7, and intestinal growth was assessed at days 19, 25, and 29 of life.<h4>Results</h4>The mean +/- SEM of T cells expressing the IL-2R during weaning (days 15-28) was 6.1% +/- 0.3% compared with 3.3% +/- 0.3% at other ages (P < 0.001). The small intestine in rats treated with blocking antibody had reduced crypt length and mitotic count compared with control animals.<h4>Conclusions</h4>Weaning is associated with activation of T cells and blockade of the IL-2R reduces intestinal growth.
Keywords: Intestinal Mucosa; Intestine, Small; Epithelium; T-Lymphocytes; Animals; Rats, Inbred Strains; Rats; Dermatitis, Contact; Receptors, Interleukin-2; Antibodies, Monoclonal; Flow Cytometry; Linear Models; Lymphocyte Activation; Weaning; Female; Male
RMID: 0030004234
DOI: 10.1053/gast.1996.v111.pm8698223
Appears in Collections:Microbiology and Immunology publications

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